Periodic Reporting for period 3 - SIMBA (Sustainable innovation of microbiome applications in food system)
Período documentado: 2021-11-01 hasta 2022-10-31
The main aim of SIMBA is to tackle the growing challenge of supplying food to meet the global population demands within a changing climate, through innovative solutions utilising microorganisms in our food system. Now more than ever, amidst the global uncertainty of the COVID-19 pandemic, we need to utilise innovative ideas to promote sustainable food production, and the microbiome can offer a possible alternative to intensive and unsustainable agriculture practices.
The SIMBA project brings together 21 partners from across the European continent. SIMBA is comprised of leading research groups from the terrestrial, marine, food and human gut microbiomes fields. The consortium forms a well-balanced mix between fundamental scientists with expertise in ecology and microbiome evolution, and applied scientists who bridge fundamental science to applications in the food industry. A number of small to medium enterprises (SME) within the consortium are key to testing novel techniques and provide “near to market” solutions for field testing and economical evaluation. This cross-sectoral collaboration ensures that the results of fundamental research will be exploited by the consortium and transferred to the market.
Marine macro-and microalgae were extensively studied with respect to the natural associated microbiomes and several potential modulating bacterial strains were isolates and are being tested to serve as growth promoting microbiomes. Microalgae were selected and tested for their nutritional value and use in fish feed. Saline agriculture of potatoes was performed inhouse and amendments with bacterial isolates and whole microbiome transplants were performed. Increased potato yield was observed for some microbiomes, that are now subject to molecular analysis to identify plant growth promoting genes and gene products.
Studies on side streams in circular economy has focused on tomato, potato and maize. Selected pulses, soy and rapeseed meal were fermented to develop new products and ingredients. In pulse-based raw-materials, fermentations of pea were continued in a bioreactor. A set of analyses, including oligosaccharides, protein degradation, peptides, vitamin B12 and K, were performed. For studying gastrointestinal digestion of fermented foods, static in vitro simulation was used for fermented pulses and samples were collected for analyses. During the current reporting period the work on human health consisted: i) The trial studying the effects of a fermented canola-seaweed product in human subjects suffering from metabolic syndrome has been completed ii) Cell culture studies testing the major polyphenol and bile acid metabolites on immune cell differentiation and gut epithelial barrier function were carried out to obtain insights into the mechanisms whereby diet-derived and microbiota-modified compounds affect metabolic health. To address the readiness to produce of selected micro-organisms in a bioreactor was established and technical procedure for pilot scale starter production and pea fermentation was drafted. Of the soil microbes, complex PGPM were designed and a pilot-scale fermentations was carried out to test various cultivation conditions. For typing of relevant micro-organisms to be used in specific applications, collection of protocols to be used for rapid identification of microbes was carried out. The environmental and social evaluation of sustainability focused on using microbial application during fish production; the economic evaluation focus on using these innovations during potato (starch and seed) and wheat production. The overall sustainability evaluation used a quantitative assessment for potato production and a qualitative evaluation for tomato production.
• Enhanced consistency of microbiomes in field applications through the identification of best mixtures of plant growth-promoting microorganisms and bio-active compounds.
• Improved understanding of the role of salt-tolerant microbiomes and their role in the cultivation of salt resistant crops, contributing to the reclaiming of land lost to desertification and increasing arable land.
• Starter cultures to be applied in food products with beneficial compounds, contributing to health human benefits.
• Improved understanding of the functions of individual and sustainable diet-induced variations in gut microbiota, and their interaction with diet and foods, which is expected to contribute to the prevention of certain chronic diseases, such as Type 2 diabetes.
• Near to market ready development of new and cost-effective applications, products and services of new microbes, food, crop and algae products through upscaling from laboratory to pilot scale processes.
• Proven increase in sustainability of European food systems by implementing the microbial interventions.
Potential impacts
• Knowledge and applications of terrestrial, marine, and food driven microbiomes to increase productivity, quality, safety, sustainability and nutrition security in the food chain
• Nutrition security can be better ensured adopting a food system approach based on sustainability, linking land and sea and encompassing the entire food value chain
• Public and private food system stakeholders can increase their innovation capacities and facilitate their access to contemporary scientific knowledge