Artificial Cells with Distributed Cores to Decipher Protein Function

Project Information

ACDC

Grant agreement ID: 824060

DOI 10.3030/824060

Project closed

EC signature date 23 October 2018

Start date 1 January 2019

End date 31 July 2023

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost € 4 376 295,00

EU contribution € 4 376 295,00

Coordinated by

UNIVERSITA DEGLI STUDI DI TRENTO
Italy

Deliverables

Demonstrators, pilots, prototypes (2)

2-phase, x-core L2 capsules
x-phase, x-core L2 capsules

xphase xcore L2 capsules

Documents, reports (16)

Simulations in ethical and societal consideration
Toolkits 1 and 2
First version of compiler
Application and refinement of optimized compiler
Ethics report
Summer School in Trento & Open Day

Summer School in Trento Open Day

Control of capsule-capsule assembly

Control of capsulecapsule assembly

Specific DNA labelling
Synthetic IL-2RA in ACDC vesicles

Synthetic IL2RA in ACDC vesicles

Potential of autocatalytic networks
Community Seeds Report
The DNA-governed assembly of capsules

The DNAgoverned assembly of capsules

Type(s) of simulation platform to be implemented
Energisation and conversion to ATP
Membrane mediated communication
Exp model of ion transport

Other (2)

Refinement of ACDC simulation platform
Implementation of first version of simulation platform

Websites, patent fillings, videos etc. (1)

Project launch

Open Research Data Pilot (1)

Data management plan

Publications

Peer reviewed articles (8)

Droplet Microfluidics for Tumor Drug-Related Studies and Programmable Artificial Cells

Author(s): Pantelitsa Dimitriou, Jin Li, Giusy Tornillo, Thomas McCloy, and David Barrow
Published in: Global Challenges, Issue Global Challenges2021, 5, 2000123, 2021, ISSN 2056-6646
Publisher: Wiley-VCH GmbH, Weinheim
DOI: 10.1002/gch2.202000123

Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies

Author(s): Baxani, D. K., Jamieson, W. D., Barrow D.A., Castell O.K.
Published in: Soft Matter, Issue 27, 2022, ISSN 2048-769X
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sm01111a

"Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis """

Author(s): Adriano Caliari, Martin M. Hanczyc, Masayuki Imai, Jian Xu, Tetsuya Yomo
Published in: Int. J. Mol. Sci, Issue 24(9, 2023, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24098241

Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells

Author(s): Patrick Hardinge, Divesh K. Baxani2,4, Thomas McCloy2,3, JamesA. H. Murray1 & Oliver K. Castell2
Published in: Nature scientific reports, Issue 10, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-78996-7

Population-level Membrane Diversity Triggers Growth and Division of Protocells

Author(s): Ö. Duhan Toparlak; Anna Wang; Sheref S. Mansy; Sheref S. Mansy
Published in: JACS Au, Vol 1, Iss 5, Pp 560-568 (2021), Issue 2, 2020, ISSN 2573-2293
Publisher: ChemRxiv
DOI: 10.26434/chemrxiv.13238402

Hybrid organic–inorganic structures trigger the formation of primitive cell-like compartments

Author(s): Holler Silvia, Stuart Bartlett, Richard Loeffler, Federica Casiraghi, Julyan Cartwright, Claro Ignacio Sainz Diaz and Martin M. Hanczyc
Published in: PNAS, Issue issue 120, vol 33, 2023, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2300491120

Development and evaluation of a low‐cost lego 3d bioprinter: from building‐blocks to building blocks of life

Author(s): Ahmad Moukachar, Katie Harvey, Eva Roke, Katherine Sloan, Cameron Pool, Shabbir Moola, Ameer Alshukri, Danielle Jarvis, Phoebe Crews‐Rees, Grace McDermott, Lluan Evans, Jin Li, Christopher Thomas, Sion Coulman, Oliver Castell
Published in: Adv.Mater. Technoll., Issue 8, 2023, ISSN 2365-709X
Publisher: Wiley-VCH Gmb
DOI: 10.1002/admt.202100868

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

Author(s): Jin Li, William,D. Jamieson, Pantelitsa Dimitriou, Wen Xu, Paul Rohde, Boris Martinac, Matthew Baker, Bruce W. Drinkwater, Oliver K. Castell, David A. Barrow
Published in: Nat Commun, Issue 13, 4125 (, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-31898-w

Conference proceedings (17)

Computational investigation of the clustering of droplets in widening pipe geometries

Author(s): Matuttis, HG. et al. (2023).
Published in: De Stefano, C., Fontanella, F., Vanneschi, L. (eds) Artificial Life and Evolutionary Computation. WIVACE 2022. Communications in Computer and Information Science, vol 1780., 2023
Publisher: Springer, Cham
DOI: 10.1007/978-3-031-31183-3_7

Ethical aspects of computational modelling in science, decision support and communication

Author(s): Füchslin, R.M. et al. (2022)
Published in: Schneider, J.J., Weyland, M.S., Flumini, D., Füchslin, R.M. (eds) Artificial Life and Evolutionary Computation. WIVACE 2021. Communications in Computer and Information Science, vol 1722., 2023, ISBN 978-3-031-23929-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-27312

Investigating three-dimensional arrangements of droplets

Author(s): Schneider, Johannes Josef Weyland, Mathias Sebastian Flumini, Dandolo Füchslin, Rudolf Marcel
Published in: Italian Workshop on Artificial Life and Evolutionary Computation, 2020, ISBN 978-3-030-45016-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-22123

Acompiler framework to derive microfluidic platforms for manufacturing hierarchical,compartmentalized structures that maximize yield of chemical reactions

Author(s): Mathias S. Weyland, Dandolo Flumini, Johannes J. Schneider, Rudolf M. Füchslin
Published in: ALIFE 2020: The 2020 Conference on Artificial Life, 2021
Publisher: MIT Press direct
DOI: 10.1162/isal_a_00303

Towards programmable chemistries

Author(s): Flumini, D., Weyland, M.S., Schneider, J.J., Fellermann, H., Füchslin, R.M.
Published in: Cicirelli, F., Guerrieri, A., Pizzuti, C., Socievole, A., Spezzano, G., Vinci, A. (eds) Artificial Life and Evolutionary Computation. WIVACE 2019. Communications in Computer and Information Science, vol 1200, 2020, ISBN 978-3-030-45016-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-19855

Exploring the three-dimensional arrangement of droplets

Author(s): Schneider, J.J., Weyland, M.S., Flumini, D., Füchslin, R.M. (2022)
Published in: Schneider, J.J., Weyland, M.S., Flumini, D., Füchslin, R.M. (eds) Artificial Life and Evolutionary Computation. WIVACE 2021. Communications in Computer and Information Science, vol 1722., 2023, ISBN 978-3-031-23929-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-27308

Simulations of Vesicular Distanglement

Author(s): """Peter Eggenberger Hotz, Federica Casiraghi, Johannes Josef Schneider, Mathias Sebastian Weyland, Dandolo Flumini, Martin Michael Hanczyc, and Rudolf Marcel Füchslin"""
Published in: Proceedings of the ALIFE 2022: The 2022 Conference on Artificial Life. ALIFE 2022: The 2022 Conference on Artificial Life, 2022
Publisher: MIT Press Direct
DOI: 10.21256/zhaw-27390

Geometric restrictions to the agglomeration of spherical particles

Author(s): Schneider, J.J. et al. (2022)
Published in: Schneider, J.J., Weyland, M.S., Flumini, D., Füchslin, R.M. (eds) Artificial Life and Evolutionary Computation. WIVACE 2021. Communications in Computer and Information Science, vol 1722., 2023, ISBN 978-3-031-23929-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-27310

Paths in a Network of Polydisperse Spherical Droplets

Author(s): Johannes Josef Schneider, Alessia Faggian, Silvia Holler, Federica Casiraghi, Jin Li, Lorena Cebolla Sanahuja, Hans-Georg Matuttis, Martin Michael Hanczyc, David Anthony Barrow, Mathias Sebastian Weyland, Dandolo Flumini, Peter Eggenberger Hotz, Patrik Eschle, Aitor Patiño Diaz, Rudolf Marcel Füchslin
Published in: Proceedings of the ALIFE 2022: The 2022 Conference on Artificial Life. ALIFE 2022: The 2022 Conference on Artificial Life., 2022
Publisher: MIT Press direct
DOI: 10.21256/zhaw-25985

Network creation during agglomeration processes of polydisperse and monodisperse systems of droplets

Author(s): Schneider, J.J. et al. (2023).
Published in: De Stefano, C., Fontanella, F., Vanneschi, L. (eds) Artificial Life and Evolutionary Computation. WIVACE 2022. Communications in Computer and Information Science, vol 1780., 2023
Publisher: Springer, Cham
DOI: 10.1007/978-3-031-31183-3_8

Simplified modeling of the evolution of skills in a spatially resolved environment

Author(s): Füchslin, Rudolf Marcel; Schneider, Johannes Josef; Ott, Thomas; Walker, Richard
Published in: Alife 2019 : proceedings of the Artificial Life Conference 2019, Issue Jul-2019, 2019, Page(s) 324-330, ISBN 978-0-262-35844-6
Publisher: Massachusetts Institute of Technology
DOI: 10.21256/zhaw-17919

Studying and simulating the three-dimensional arrangement of droplets

Author(s): Schneider, Johannes Josef Weyland, Mathias Sebastian Flumini, Dandolo Matuttis, Hans-Georg Morgenstern, Ingo et al.
Published in: Cicirelli, F., Guerrieri, A., Pizzuti, C., Socievole, A., Spezzano, G., Vinci, A. (eds) Artificial Life and Evolutionary Computation. WIVACE 2019. Communications in Computer and Information Science, vol 1200., 2020, ISBN 978-3-030-45016-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-19856

Obstacles on the pathway towards chemical programmability using agglomerations of droplets

Author(s): Schneider, J.J. et al. (2022
Published in: Schneider, J.J., Weyland, M.S., Flumini, D., Füchslin, R.M. (eds) Artificial Life and Evolutionary Computation. WIVACE 2021. Communications in Computer and Information Science, vol 1722, 2023, ISBN 978-3-031-23929-8
Publisher: Springer, Cham
DOI: 10.21256/zhaw-27309

Influence of the geometry on the agglomeration of a polydisperse binary system of spherical particles

Author(s): Johannes Josef Schneider, Alessia Faggian, Silvia Holler, Federica Casiraghi, Jin Li, Lorena Cebolla Sanahuja, Hans-Georg Matuttis, Martin Michael Hanczyc, David Anthony Barrow, Mathias Sebastian Weyland, Dandolo Flumini, Peter Eggenberger Hotz, Rudolf Marcel Füchslin
Published in: ALIFE 2021: The 2021 Conference on Artificial Life, 2021
Publisher: MIT Press direct
DOI: 10.1162/isal_a_00392

A Chemical Compiler for the Synthesis of Branched Oligomers on Standardized Chemical Reaction Structures

Author(s): Mathias S. Weyland, Dandolo Flumini, Johannes J. Schneider, Alessia Faggian, Aitor Patiño Diaz, Rudolf M. Füchslin
Published in: Proceedings of the ALIFE 2023: Ghost in the Machine:, 2023
Publisher: ASME
DOI: 10.21256/zhaw-28699

The good, the bad and the ugly : droplet recognition by a “shootout”-heuristics

Author(s): Matuttis, HG. et al. (2022
Published in: Schneider, J.J., Weyland, M.S., Flumini, D., Füchslin, R.M. (eds) Artificial Life and Evolutionary Computation. WIVACE 2021. Communications in Computer and Information Science, vol 1722., 2023
Publisher: Springer, Cham
DOI: 10.21256/zhaw-27307

Artificial chemistry performed in an agglomeration of droplets with restricted molecule transfer

Author(s): Schneider, J.J. et al. (2023)
Published in: De Stefano, C., Fontanella, F., Vanneschi, L. (eds) Artificial Life and Evolutionary Computation. WIVACE 2022. Communications in Computer and Information Science, vol 1780., 2023
Publisher: Springer, Cham
DOI: 10.1007/978-3-031-31183-3_9