Project description DEENESFRITPL A sensitive hormone test useful for obesity management Obesity affects more than 10 % of individuals worldwide, but there are no specific treatments. Therefore, there is a pressing need for novel diagnostic and prognostic biomarkers. The EU-funded DNAsensPROT project has developed a DNA aptamer-based method for the quantitative measurement of proteins within biological samples. The idea is to apply this approach for the measurement of leptin, a gut hormone which regulates appetite and weight and whose levels are proportional to body fat mass. The implementation of this method in a point-of-care setting offers an ultrasensitive test for the diagnosis of appetite control problems such as those encountered in obesity and anorexia. Show the project objective Hide the project objective Objective The life expectancy in both EU and US fell in 2015. A third of premature deaths could be preventable and large part is due to obesity – a major risk factor for many chronic diseases including diabetes. Worldwide about 40% of adults are overweight and 13% obese. Regulation of food metabolism is becoming one of the main issues relevant to human health today. Obesity is preventable, however there are no currently available non-invasive and cost-effective obesity treatments, pointing to the need of novel diagnostic and prognostic tests. Leptin, a gut-hormone protein, has been shown to be crucial in regulating appetite and weight. Its concentration is proportional to the body fat mass, and changes at a greater rate compared to weight loss, therefore it can be used as a key measure of diet efficiency. Strong correlation between plasma and salivary leptin levels allows monitoring leptin in saliva, and provides point-of-care (PoC) personalised insight into the success of individual weight loss programs.During our ERC Synergy Grant we have developed an aptamer based approach to perform quantitative detection of protein molecules using nucleic acid amplification to overcome the complexities of current methods to enable PoC detection. The key idea (patent pending) is to amplify and quantify DNA displaced from DNA-aptamer complexes by Leptin molecules with real-time Loop Mediated Isothermal Amplification. We will use an Ion Sensitive Field Effect Transistor lab-on chip arrays. Now we need funds to further develop this technology and create a PoC platform to provide linear label-free ultrasensitive protein detection, reducing complexity, time-to-result and cost of the assay. Furthermore, the development of this novel molecular method will enable quantification of a broader number of protein targets and create one of the most sensitive PoC techniques for protein quantification, with applications far beyond appetite control (obesity, anorexia etc) management. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineendocrinologydiabetesmedical and health scienceshealth sciencesnutritionobesity Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-2018-PoC - ERC Proof of Concept Grant Call for proposal ERC-2018-PoC See other projects for this call Funding Scheme ERC-POC - Proof of Concept Grant Coordinator IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Net EU contribution € 149 956,00 Address South kensington campus exhibition road SW7 2AZ London United Kingdom See on map Region London Inner London — West Westminster Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,25 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE United Kingdom Net EU contribution € 149 956,00 Address South kensington campus exhibition road SW7 2AZ London See on map Region London Inner London — West Westminster Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,25
