A STEP FORWARD TO SPINAL CORD INJURY REPAIR USING INNOVATIVE STIMULATED NANOENGINEERED SCAFFOLDS

Informations projet

NeuroStimSpinal

N° de convention de subvention: 829060

DOI

10.3030/829060

Projet clôturé

Date de signature de la CE 17 Decembre 2018

Date de début 1 Avril 2019

Date de fin 30 Septembre 2023

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 3 518 962,50

Contribution de l’UE

€ 3 503 922,50

Coordonné par

UNIVERSIDADE DE AVEIRO
Portugal

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (20)

Report describing the type of biological functionalization of the different scaffolds

Report describing the methodologies adopted if needed to biologically functionalize the different scaffolds in order to increase their biocompatibility and bioactivity Task 33

Communication (CP) and Dissemination (DP) plan revision

Revision of the communication and dissemination plans

Report on the Communication (CP) and Dissemination (DP) plan follow up

Final document reporting on the Communication and Dissemination plans accomplishement

Management plan and Protection

Define the Knowledge management plan and Protection of the results obtained during the project.

Report on organs toxicity

Report on the hepatotoxicity renal toxicity and pulmonary toxicity

Electrical circuit specifications

A report describing the electrical circuit specifications able to control the amount of charge the direction of its transfer its shape and the peak voltage being developed in the Task 21 will be delivered

Report on biocompatibility in vitro

Report on in vitro biocompatibility of the scaffolds in the presence of the cells under study Details about cell viability proliferation structure and differentiation assessed at different time points will be detailed results obtained under Task 42

Electronic controller board state of the art

A report describing the state of the art and current specifications about electronic controller board specificity.

Preliminary evaluation of biological functionalization of the scaffolds

Report describing the first biological functionalization of the different scaffolds produced and characterized in tasks 31 and 32

Synaptogenesis and neuritogenesis

Report on the mechanism behavior of the neurons seeded in the adECMGBM scaffolds will be delivered at the end of Task 44 This report will also include results from other tasks namely 45 and 47 due to the close interrelation between them

Growth and differentiation of primary ENPCs in the adECM/GBM scaffolds

Report describing the results obtained on the growth and differentiation of primary ENPCs in the different developed adECMGBM scaffolds under Tasks 42 and 43

Report describing the preparation of different shaped GBM/adECM scaffolds

Report describing in detail the preparation methodologies and characterization of the different shaped GBMadECM scaffolds produced These activities will be done in Tasks 31 and 32

Communication (CP) and Dissemination (DP) plan

Communication (CP) and Dissemination (DP) plan will be prepared.

Preliminary study about electrode and scaffold connection

Report describing preliminary results about the electrode connection between the device and the scaffold through a graphenebased ink developed in Task 23

Response of different neural cell lines

Report describing the response of different neural cell lines

State of the art analysis

A report describing the state of the art and current specifications about the electrical circuit foreseen for the particular applications in this project.

Device connection to the scaffold

Report describing the electrode connection between the device with the scaffold based on a graphenebased ink developed in Task 23

Report on immune response in vitro

Report on immune response in vitro studies with macrophages innate immune system and T lymphocytes adaptive response will be detailed Task 46

Preliminar report on production and characterization of scaffolds

Preliminary report describing the preparation methodologies and characterization of GBM/adECM scaffolds. These activities will be done in Tasks 3.1 and 3.2

Finalization of biocompatibility and efficacy studies in the injured SC after local exposure to the scaffold matrices in small preclinical models

Finalization of biocompatibility and efficacy studies in the injured SC after local exposure to the scaffold matrices in small preclinical models This report will bring together all results related to the in vivo studies conducted with the optimized scaffolds with and without electrical stimulation Task 53

Open Research Data Pilot (3)

Data Management plan

To comply with the Open Research data Pilot, a Data Management Plan will be prepared, which will be a dynamic document that will be updated during the project execution.

Data Management plan revision 2

Revision and update if necessary of the previously implemented and revised Data Management Plan

Data Management plan revision 1

Revision and update if necessary of the previously implemented Data Management Plan

Websites, patent fillings, videos etc. (1)

NeuroStimSpinal logo and website

The NeuroStimSpinal logo and Website will be created.

Other (3)

Workshop organization 2

A second and final workshop will be organized dedicated to the thematic of the NeuroStimSpinal project. Experts in the field will be invited to participate and all the project members will present results and conclusions of the project.

ENPCs isolation

Deliver of the isolated ENPCs necessary for subsequent tasks

Workshop organization 1

A first workshop will be organized dedicated to the thematic of the NeuroStimSpinal project Experts in the field will be invited to participate and all the project members will present ongoing results

Publications

Peer reviewed articles (19)

Customizing 3D Structures of Vertically Aligned Carbon Nanotubes to Direct Neural Stem Cell Differentiation

Auteurs: Marques, Paula
Publié dans: Advanced Healthcare Materials, Numéro 7, 2023, Page(s) 2300828 (1 of 15), ISSN 2192-2659
Éditeur: World Scientific
DOI: 10.1002/adhm.202300828

Effects of graphene oxide and reduced graphene oxide nanomaterials on porcine endothelial progenitor cells

Auteurs: Alberto Polo-Montalvo, Mónica Cicuéndez, Laura Casarrubios, Nathalie Barroca, Daniela da Silva, María José Feito, Rosalía Diez-Orejas, María Concepción Serrano, Paula A. A. P. Marques and María Teresa Portolés
Publié dans: Nanoscale, 2023, Page(s) 17173-17183, ISSN 2040-3372
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d3nr03145d

Is Graphene Shortening the Path toward Spinal Cord Regeneration?

Auteurs: André F. Girão, María Concepcion Serrano, António Completo, and Paula A. A. P. Marques
Publié dans: ACS Nano, 2022, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.2c04756

Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering

Auteurs: Marques, Paula
Publié dans: Biomaterials Advances, Numéro 22, 2023, Page(s) 213351, ISSN 2772-9508
Éditeur: Elsevier
DOI: 10.1016/j.bioadv.2023.213351

Decellularized extracellular matrix-based 3D nanofibrous scaffolds functionalized with polydopamine-reduced graphene oxide for neural tissue engineering

Auteurs: Marques, Paula
Publié dans: Chemical Engineering Journal, Numéro 20, 2023, Page(s) 144980, ISSN 1873-3212
Éditeur: Elsevier
DOI: 10.1016/j.cej.2023.144980

Amniotic Membrane‐Derived Multichannel Hydrogels for Neural Tissue Repair

Auteurs: Joana P.M. Sousa, Inês A. Deus, Cátia F. Monteiro, Catarina A. Custódio, João Gil, Lina Papadimitriou, Anthi Ranella, Emmanuel Stratakis, João F. Mano, Paula A.A.P. Marques
Publié dans: Advanced Healthcare Materials, 2024, ISSN 2192-2640
Éditeur: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202400522

Comparative analysis of aligned and random amniotic membrane-derived cryogels for neural tissue repair

Auteurs: Joana P. M. Sousa, Inês A. Deus, Cátia F. Monteiro, Catarina A. Custódio, Emmanuel Stratakis, João F. Mano, Paula A. A. P. Marques
Publié dans: Biomaterials Science, Numéro 12, 2024, Page(s) 4393-4406, ISSN 2047-4830
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d4bm00364k

The role of mechanobiology on the Schwann cell response: A tissue engineering perspective

Auteurs: Phanee Manganas, Paraskevi Kavatzikidou, Antonis Kordas, Eleftheria Babaliari, Emmanuel Stratakis and Anthi Ranella
Publié dans: Frontiers in Celluluar Neuroscience, 2022, ISSN 1662-5102
Éditeur: Frontiers Research Foundation
DOI: 10.3389/fncel.2022.948454

Benefits in the Macrophage Response Due to Graphene Oxide Reduction by Thermal Treatment

Auteurs: Cicuéndez, M.; Casarrubios, L.; Barroca, N.; Silva, D.; Feito, M.J.; Diez‐Orejas, R.; Marques, P.A.A.P.; Portolés, M.T.
Publié dans: International Journal of Molecular Science, 2021, Page(s) 6701 (16 pages), ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22136701

Systematic Evaluation of Spinal Cord Injury Animal Models in the Field of Biomaterials

Auteurs: Kest Verstappen, René Aquarius, Alexey Klymov, Kimberley Wever, Lyan Damveld, Sander C.G. Leeuwenburgh, Ronald H.M.A. Bartels, Carlijn R Hooijmans, X Frank Walboomers
Publié dans: Tissue Engineering Part B: Reviews, 2021, ISSN 1937-3368
Éditeur: Mary Ann Liebert Inc.
DOI: 10.1089/ten.teb.2021.0194

Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications

Auteurs: Kest Verstappen, Alexey Klymov, Mónica Cicuéndez, Daniela M. da Silva, Nathalie Barroca, Francisco-Javier Fernández-San-Argimiro, Iratxe Madarieta, Laura Casarrubios, María José Feito, Rosalía Diez-Orejas, Rita Ferreira, Sander C.G. Leeuwenburgh, María Teresa Portolés, Paula A.A.P. Marques, X. Frank Walboomers
Publié dans: Materials Today Bio, Numéro 26, 2024, Page(s) 101059, ISSN 2590-0064
Éditeur: Elsevier
DOI: 10.1016/j.mtbio.2024.101059

Combined effect of shear stress and laser-patterned topography on Schwann cell outgrowth: synergistic or antagonistic?

Auteurs: Babaliari, E.; Kavatzikidou, P.; Mitraki, A.; Papaharilaou, Y.; Ranella, A.; Stratakis, E.
Publié dans: Biomaterials Science, Numéro 7, 2020, Page(s) 1334-1344, ISSN 2047-4849
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0bm01218a

Candida albicans/Macrophage Biointerface on Human andPorcine Decellularized Adipose Matrices

Auteurs: Mónica Cicuéndez, Laura Casarrubios, María José Feito, Iratxe Madarieta, Nerea Garcia-Urkia, Olatz Murua, Beatriz Olalde, Nerea Briz, Rosalía Diez-Orejas, María Teresa Portolés
Publié dans: Journal of Fungi, Numéro 1, 2021, Page(s) 392, ISSN 2309-608X
Éditeur: MDPI
DOI: 10.3390/jof7050392

Biofabrication for neural tissue engineering applications

Auteurs: L. Papadimitriou, P. Manganas, A. Ranella, E. Stratakis
Publié dans: Materials Today Bio, Numéro 6, 2020, Page(s) 100043, ISSN 2590-0064
Éditeur: Elsevier
DOI: 10.1016/j.mtbio.2020.100043

Functionality of macrophages encapsulated in porcine decellularized adipose matrix hydrogels and interaction with Candida albicans

Auteurs: Mónica Cicuéndez, Andrea García-Lizarribar, Laura Casarrubios, María José Feit, Francisco Javier Fernández-San-Argimiro, Nerea García-Urkia, Olatz Murua, Iratxe Madarieta, Beatriz Olalde, Rosalía Diez-Orejas, María Teresa Portolés
Publié dans: Biomaterials Advances, Numéro Volume 159, 2024, Page(s) 213794, ISSN 2772-9508
Éditeur: Elsevier
DOI: 10.1016/j.bioadv.2024.213794

Magnetoresponsive Anisotropic Fiber‐Integrating Hydrogels for Neural Tissue Regeneration

Auteurs: Joana P. M. Sousa, Cátia F. Monteiro, Inês A. Deus, António Completo, Emmanuel Stratakis, João F. Mano, Paula A. A. P. Marques
Publié dans: Small Structures, 2024, ISSN 2688-4062
Éditeur: Wiley
DOI: 10.1002/sstr.202400213

Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence

Auteurs: Cicuéndez, M., Casarrubios, L., Feito, M.J., Madarieta, I., Garcia-Urkia, N., Murua, O., Olalde, B. Briz, N., Diez-Orejas, R., Portolés, M.T.
Publié dans: International Journal of Molecular Sciences, Numéro 1, 2021, Page(s) 3847, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22083847

Effects of Graphene Oxide and Reduced Graphene Oxide Nanostructures on CD4+ Th2 Lymphocytes

Auteurs: María José Feito, Mónica Cicuéndez, Laura Casarrubios, Rosalía Diez-Orejas, Sara Fateixa, Daniela Silva, Nathalie Barroca, Paula A. A. P. Marques, María Teresa Portolés
Publié dans: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms231810625

Anisotropic 3D scaffolds for spinal cord guided repair: Current concepts

Auteurs: Marques, Paula
Publié dans: Biomaterials Advances, Numéro 9, 2023, Page(s) 213353, ISSN 2772-9508
Éditeur: Elsevier
DOI: 10.1016/j.bioadv.2023.213353

Monographic books (1)

TEchMA2022 – 5th International Conference on Technologies for the Wellbeing and Sustainable Manufacturing Solutions: Book of abstracts

Auteurs: Pereira, António; Marques, Paula; Coelho, Margarida; Completo, António; Neto, Fernando
Publié dans: Numéro 22, 2022
Éditeur: University of Aveiro
DOI: 10.48528/2smr-sn15

Conference proceedings (3)

Circuit Modeling of rGO-doped Scaffolds for Spinal Cord Regeneration Based on Transient and xAC Analyses

Auteurs: Latifah Al-Maghrabi; Patrícia Martins; Daniela Silva; Guilherme Gil; Nathalie Barroca; Olatz Murua; Beatriz Olalde; Luís Alves; Paulo Pedreiraa, Pedro Fonseca, Philip LeDuc, Paula Marques
Publié dans: IEEE International Symposium on Circuits and Systems (ISCAS), 2022, ISBN 978-1-6654-8485-5
Éditeur: IEEE
DOI: 10.1109/iscas48785.2022.9937411

TEchMA2021: 4th International Conference on Technologies for the Wellbeing and Sustainable Manufacturing Solutions: book of abstracts

Auteurs: Pereira, António; Marques, Paula; Coelho, Margarida; Completo, António; Neto, Fernando
Publié dans: TEchMA2021: 4th International Conference on Technologies for the Wellbeing and Sustainable Manufacturing Solutions: book of abstracts, Numéro 9, 2021, Page(s) 27 and 30, ISBN 978-972-789-667-7
Éditeur: UA Editora, Universidade de Aveiro
DOI: 10.34624/7q83-ay77

A multimaterial multifunctional patch to repair thespinal cord contusion injury

Auteurs: Daniela Silva, Joana Couto, Ângela Semitela, Susana C. Pinto, Sandra I. Vieira, Paula A.A.P. Marques, Nathalie Barroca
Publié dans: TEchMA 2023– 6th International Conference on Technologies for the Wellbeing and Sustainable Manufacturing Solutions, 2023, Page(s) 65, ISBN 978-972-789-858-9
Éditeur: UA Editora Universidade de Aveiro
DOI: 10.48528/tspw-yj21