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Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation

Deliverables

Toolbox of MNPs V3.0/Public

Public report on final round of MNPs toolbox with magnetic heating profiles complementary to the Topt of the enzymes

Report MNP general toxicity

Report on MNPs toxicity

Third round of project newsletters

Report on the preparation of the third round of Newsletters

Final report MNP general toxicity

Final report on MNPs toxicity

Monoenzyme hybrid composite toolbox/Public

Public report on the proof of concept of hybrid composites bearing one enzyme immobilized on MNPs entrapped into 3D-porous microparticles

Hybrid composites stability at operational conditions for sugar biotransformations

Report on hybrids stability for hightemperature sugar biotransformations under extensive AMF use

Cell free system for enzyme synthesis/immobilization_Public

Public report on cellfree system to synthesize and immobilized enzyme variants in onepot

Second round of project newsletters

Report on the preparation of the second round of Newsletters

Industrial Partnering event

Standalone event with potencial industrial stakeholders

Assay for metals ion leakage detection

Assay to detect metal ions leakage from MNPs

Hybrid stability at AMF operational conditions for THIQ production

Report on the hybrid composites stability for THIQ production under extensive AMF use

Leaflets and rollers

Report on the production of Leaflets and rollers

AMF features to tune enzyme activity/Public

Public report on optimal AMF settings for tuning ADHT activity by reaching different local temperatures using the selected MNPs at D1.1

Hybrid composites stability and toxicity

Stability of the different hybrid composites under extensive AMF use and toxicity tests

Enzyme@MNP stability and toxicity

Stability of the different engineered enzymes immobilized on MNPs under extensive AMF use and toxicity tests

Hotzyme website, logo and social media accounts

Report on Hotzyme website, logo and social media accounts (i.e: Tweeter, Facebook and LinkedIn)

Functionalized MNP toolbox

MNPs with complementary chemistry to immobilize the engineered enzymes

AMF features to tune heat transfer V2.0/Public

Public final report on features that allow tuning MNPs heat transfer

Final round of project newsletters

Report on the preparation of the final round of Newsletters

Multienzyme hybrid composite toolbox V2.0_Public

Public report on final multienzyme hybrid composite toolbox simultaneously or sequentially activated under certain AMF settings

First round of project newsletters

Report on the preparation of the first round of Newsletters

Thermal nanoactuated synthesis of α-glucosyl-2-glycerate_Public

Public report on new AMFtuned process window for hightemperature synthesis of glucosyl2glycerate

Aminoacid cluster toolbox/Public

Public report on aminoacid cluster toolbox to allow enzyme orientation through target regions

Thermal nanoactuated sucrose conversion wo fructose decomposition_Public

Public report on new AMFtuned process window for hightemperature conversion of sucrose without fructose breakdown

His-tagged enzymes variants

His-tagged variants to immobilized enzyme through N- or C-terminus

Publications

Reproducibility and scalability of magnetic nanoheater synthesis

Author(s): Ovejero, Jesus; Gallo-Cordova, Alvaro; Roca, Alejandro G.; Morales, M.P.; Veintimillas-Verdaguer, Sabino
Published in: Nanomaterials, Issue 20794991, 2021, ISSN 2079-4991
Publisher: MDPI AG
DOI: 10.3390/nano11082059

The Yin and Yang of epigenetics in the field of nanoparticles

Author(s): Elettra Musolino; Christina Pagiatakis; Simone Serio; Marina Borgese; Federica Gamberoni; Rosalba Gornati; Giovanni Bernardini; Roberto Papait
Published in: Nanoscale Advances, Issue 25160230, 2022, ISSN 2516-0230
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1na00682g

Mixing iron oxide nanoparticles with different shape and size for tunable magneto-heating performance

Author(s): Jesus G. Ovejero, Federico Spizzo, M. Puerto Morales, Lucia Del Bianco
Published in: Nanoscale, Issue 13/11, 2021, Page(s) 5714-5729, ISSN 2040-3364
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0nr09121a

How size, shape and assembly of magnetic nanoparticles give rise to different hyperthermia scenarios

Author(s): Gavilan, H.; Simeonidis, K.; Myrovali, E.; Mazarío, E.; Chubykalo-Fesenko, O.; Chantrell, R.; Balcells, Ll.; Angelakeris, M; Morales, M.P.; Serantes, D.
Published in: Nanoscale, Issue 20403364, 2021, ISSN 2040-3364
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1nr03484g

Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles.

Author(s): Raquel Moreno-Loshuertos; Ángel Millán; P. Tellez; Patricio Fernández-Silva; Luis Martínez-Lostao; Carlos D. S. Brites; Rafael Piñol; Albano N. Carneiro Neto; A. Martinez; Yuanyu Gu; Justyna Zeler; Justyna Zeler; Thiago Silva; Thiago Silva; Ana Isabel Gallego; Luís D. Carlos
Published in: Nano Letters, Issue 3, 2020, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.0c02163

Tailoring the Magnetic and Structural Properties of Manganese/Zinc Doped Iron Oxide Nanoparticles through Microwaves-Assisted Polyol Synthesis

Author(s): Porru, Margherita; Morales, Maria del Puerto; Gallo-Cordova, Alvaro; Espinosa, Ana; Moros, Maria; Brero, Francesca; Mariani, Manuel; Lascialfari, Alessandro; G. Ovejero, Jesus
Published in: Nanomaterials, Issue 20794991, 2022, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano12193304

Magnetic hyperthermia with ε-Fe 2 O 3 nanoparticles

Author(s): Yuanyu Gu, Marie Yoshikiyo, Asuka Namai, Debora Bonvin, Abelardo Martinez, Rafael Piñol, Pedro Téllez, Nuno J. O. Silva, Fredrik Ahrentorp, Christer Johansson, Joaquín Marco-Brualla, Raquel Moreno-Loshuertos, Patricio Fernández-Silva, Yuwen Cui, Shin-ichi Ohkoshi, Angel Millán
Published in: RSC Advances, Issue 10/48, 2020, Page(s) 28786-28797, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0ra04361c

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

Author(s): Alejandro H. Orrego, Daniel Andrés-Sanz, Susana Velasco-Lozano, Mercedes Sanchez-Costa, José Berenguer, José M. Guisan, Javier Rocha-Martin, Fernando López-Gallego
Published in: Catalysis Science & Technology, 2021, ISSN 2044-4753
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1cy00268f

Photonic and magnetic materials for on-demand local drug delivery

Author(s): Armenia, Ilaria; Cuestas Ayllon, Carlos; Torres Herrero, Beatriz; Bussolari, Francesca; Alfranca, Gabriel; Grazú, Valeria; Martínez de la Fuente, Jesús
Published in: Advanced Drug Delivery Reviews, Issue 0169409X, 2022, ISSN 0169-409X
Publisher: Elsevier BV
DOI: 10.1016/j.addr.2022.114584

Antimicrobial Activity of Nanoconjugated Glycopeptide Antibiotics and Their Effect on Staphylococcus aureus Biofilm

Author(s): Berini, Francesca; Orlandi, Viviana Teresa; Gamberoni, Federica; Martegani, Eleonora; Armenia, Ilaria; Gornati, Rosalba; Bernardini, Giovanni; Marinelli, Flavia
Published in: Frontiers in Microbiology, Issue 1664302X, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.657431

Continuous production of magnetic iron oxide nanocrystals by oxidative precipitation

Author(s): T. Asimakidou, A. Makridis, S. Veintemillas-Verdaguer, M.P. Morales, I. Kellartzis, M. Mitrakas, G. Vourlias, M. Angelakeris, K. Simeonidis
Published in: Chemical Engineering Journal, Issue 393, 2020, Page(s) 124593, ISSN 1385-8947
Publisher: Elsevier BV
DOI: 10.1016/j.cej.2020.124593

Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles

Author(s): Patrizia Bonfanti, Anita Colombo, Melissa Saibene, Luisa Fiandra, Ilaria Armenia, Federica Gamberoni, Rosalba Gornati, Giovanni Bernardini, Paride Mantecca
Published in: Nanotoxicology, Issue 14/2, 2020, Page(s) 196-213, ISSN 1743-5390
Publisher: Informa Healthcare
DOI: 10.1080/17435390.2019.1685695

Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports

Author(s): Sonali Correa, Magdalena Ripoll, Erienne Jackson, Valeria Grazú, Lorena Betancor
Published in: Catalysts, Issue 10/6, 2020, Page(s) 669, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal10060669

The Thermodynamics of Medial Vascular Calcification

Author(s): Ángel Millán, Peter Lanzer, Víctor Sorribas
Published in: Frontiers in cell and developmental biology, 2021, ISSN 2296-634X
Publisher: Frontiers Media S.A.
DOI: 10.3389/fcell.2021.633465

Efficient Brownian oscillators and nanoheaters based on gallium-doped ε-Fe2O3

Author(s): Yuanyu Gu, Nuno J. O. Silva, Marie Yoshikiyo, Asuka Namai, Rafael Piñol, Guillaume Maurin-Pasturel, Yuwen Cui, Shin-ichi Ohkoshi, Angel Millán ORCID, Abelardo Martínez
Published in: Chemical Communications, 2021, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cc07309a

High-Dose Exposure to Polymer-Coated Iron Oxide Nanoparticles Elicits Autophagy-Dependent Ferroptosis in Susceptible Cancer Cells

Author(s): Thanpisit Lomphithak, Selin Helvacioglu, Ilaria Armenia, Sandeep Keshavan, Jesus g. Ovejero, Giovanni Baldi, Costanza Ravagli, Valeria Grazu, Bengt Fadeel
Published in: Nanomaterials, Issue 13(11), 2023, Page(s) 1719, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano13111719

Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?

Author(s): Berini, Francesca; Orlandi, Viviana; Gornati, Rosalba; Bernardini, Giovanni; Marinelli, Flavia
Published in: Biotechnology Advances, Issue 07349750, 2022, ISSN 0734-9750
Publisher: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107948

Nanoparticles for Magnetic Heating: When Two (or More) Is Better Than One

Author(s): Jesus G. Ovejero; Federico Spizzo; M. Puerto Morales; Lucia Del Bianco
Published in: Materials, Issue 20782489, 2021, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ma14216416

Selective Magnetic Nanoheating: Combining Iron Oxide Nanoparticles for Multi-Hot-Spot Induction and Sequential Regulation.

Author(s): Jesus G. Ovejero; Ilaria Armenia; David Serantes; Sabino Veintemillas-Verdaguer; Nicoll Zeballos; Fernando López-Gallego; Cordula Grüttner; Jesús M. de la Fuente; María del Puerto Morales; Valeria Grazú
Published in: Nano Letters, Issue 15306984, 2021, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.1c02178

Energetics of the Glycosyl Transfer Reactions of Sucrose Phosphorylase

Author(s): Anisha Vyas, Bernd Nidetzky
Published in: Biochemistry, 2023, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.3c00080

Understanding MNPs Behaviour in Response to AMF in Biological Milieus and the Effects at the Cellular Level: Implications for a Rational Design That Drives Magnetic Hyperthermia Therapy toward Clinical Implementation.

Author(s): David Egea-Benavente; Jesus G. Ovejero; Jesus G. Ovejero; María del Puerto Morales; Domingo F. Barber
Published in: Cancers, Vol 13, Iss 4583, p 4583 (2021), Issue 20726694, 2021, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13184583

Magnetic Iron Oxide Colloids for Environmental Applications

Author(s): Alvaro Gallo-Cordova, Daniela Almeida Streitwieser, María del Puerto Morales, Jesús G. Ovejero
Published in: Colloids - Types, Preparation and Applications [Working Title], 2021
Publisher: IntechOpen
DOI: 10.5772/intechopen.95351

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