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Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation

Deliverables

Report MNP general toxicity

Report on MNPs toxicity

Monoenzyme hybrid composite toolbox/Public

Public report on the proof of concept of hybrid composites bearing one enzyme immobilized on MNPs entrapped into 3D-porous microparticles

Second round of project newsletters

Report on the preparation of the second round of Newsletters

Assay for metals ion leakage detection

Assay to detect metal ions leakage from MNPs

Leaflets and rollers

Report on the production of Leaflets and rollers

AMF features to tune enzyme activity/Public

Public report on optimal AMF settings for tuning ADHT activity by reaching different local temperatures using the selected MNPs at D1.1

Hotzyme website, logo and social media accounts

Report on Hotzyme website, logo and social media accounts (i.e: Tweeter, Facebook and LinkedIn)

Functionalized MNP toolbox

MNPs with complementary chemistry to immobilize the engineered enzymes

First round of project newsletters

Report on the preparation of the first round of Newsletters

Aminoacid cluster toolbox/Public

Public report on aminoacid cluster toolbox to allow enzyme orientation through target regions

His-tagged enzymes variants

His-tagged variants to immobilized enzyme through N- or C-terminus

Searching for OpenAIRE data...

Publications

Mixing iron oxide nanoparticles with different shape and size for tunable magneto-heating performance

Author(s): Jesus G. Ovejero, Federico Spizzo, M. Puerto Morales, Lucia Del Bianco
Published in: Nanoscale, Issue 13/11, 2021, Page(s) 5714-5729, ISSN 2040-3364
DOI: 10.1039/d0nr09121a

Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles.

Author(s): Raquel Moreno-Loshuertos; Ángel Millán; P. Tellez; Patricio Fernández-Silva; Luis Martínez-Lostao; Carlos D. S. Brites; Rafael Piñol; Albano N. Carneiro Neto; A. Martinez; Yuanyu Gu; Justyna Zeler; Justyna Zeler; Thiago Silva; Thiago Silva; Ana Isabel Gallego; Luís D. Carlos
Published in: Nano Letters, Issue 3, 2020, ISSN 1530-6984
DOI: 10.1021/acs.nanolett.0c02163

Magnetic hyperthermia with ε-Fe 2 O 3 nanoparticles

Author(s): Yuanyu Gu, Marie Yoshikiyo, Asuka Namai, Debora Bonvin, Abelardo Martinez, Rafael Piñol, Pedro Téllez, Nuno J. O. Silva, Fredrik Ahrentorp, Christer Johansson, Joaquín Marco-Brualla, Raquel Moreno-Loshuertos, Patricio Fernández-Silva, Yuwen Cui, Shin-ichi Ohkoshi, Angel Millán
Published in: RSC Advances, Issue 10/48, 2020, Page(s) 28786-28797, ISSN 2046-2069
DOI: 10.1039/d0ra04361c

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

Author(s): Alejandro H. Orrego, Daniel Andrés-Sanz, Susana Velasco-Lozano, Mercedes Sanchez-Costa, José Berenguer, José M. Guisan, Javier Rocha-Martin, Fernando López-Gallego
Published in: Catalysis Science & Technology, 2021, ISSN 2044-4753
DOI: 10.1039/d1cy00268f

Continuous production of magnetic iron oxide nanocrystals by oxidative precipitation

Author(s): T. Asimakidou, A. Makridis, S. Veintemillas-Verdaguer, M.P. Morales, I. Kellartzis, M. Mitrakas, G. Vourlias, M. Angelakeris, K. Simeonidis
Published in: Chemical Engineering Journal, Issue 393, 2020, Page(s) 124593, ISSN 1385-8947
DOI: 10.1016/j.cej.2020.124593

Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles

Author(s): Patrizia Bonfanti, Anita Colombo, Melissa Saibene, Luisa Fiandra, Ilaria Armenia, Federica Gamberoni, Rosalba Gornati, Giovanni Bernardini, Paride Mantecca
Published in: Nanotoxicology, Issue 14/2, 2020, Page(s) 196-213, ISSN 1743-5390
DOI: 10.1080/17435390.2019.1685695

Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports

Author(s): Sonali Correa, Magdalena Ripoll, Erienne Jackson, Valeria Grazú, Lorena Betancor
Published in: Catalysts, Issue 10/6, 2020, Page(s) 669, ISSN 2073-4344
DOI: 10.3390/catal10060669