Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation

Informations projet

HOTZYMES

N° de convention de subvention: 829162

DOI

10.3030/829162

Projet clôturé

Date de signature de la CE 17 Decembre 2018

Date de début 1 Avril 2019

Date de fin 30 Septembre 2022

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 2 992 388,75

Contribution de l’UE

€ 2 992 388,75

Coordonné par

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (25)

Toolbox of MNPs V3.0/Public

Public report on final round of MNPs toolbox with magnetic heating profiles complementary to the Topt of the enzymes

Report MNP general toxicity

Report on MNPs toxicity

Third round of project newsletters

Report on the preparation of the third round of Newsletters

Final report MNP general toxicity

Final report on MNPs toxicity

Monoenzyme hybrid composite toolbox/Public

Public report on the proof of concept of hybrid composites bearing one enzyme immobilized on MNPs entrapped into 3D-porous microparticles

Hybrid composites stability at operational conditions for sugar biotransformations

Report on hybrids stability for hightemperature sugar biotransformations under extensive AMF use

Cell free system for enzyme synthesis/immobilization_Public

Public report on cellfree system to synthesize and immobilized enzyme variants in onepot

Second round of project newsletters

Report on the preparation of the second round of Newsletters

Industrial Partnering event

Standalone event with potencial industrial stakeholders

Assay for metals ion leakage detection

Assay to detect metal ions leakage from MNPs

Hybrid stability at AMF operational conditions for THIQ production

Report on the hybrid composites stability for THIQ production under extensive AMF use

Leaflets and rollers

Report on the production of Leaflets and rollers

AMF features to tune enzyme activity/Public

Public report on optimal AMF settings for tuning ADHT activity by reaching different local temperatures using the selected MNPs at D1.1

Hybrid composites stability and toxicity

Stability of the different hybrid composites under extensive AMF use and toxicity tests

Enzyme@MNP stability and toxicity

Stability of the different engineered enzymes immobilized on MNPs under extensive AMF use and toxicity tests

Hotzyme website, logo and social media accounts

Report on Hotzyme website, logo and social media accounts (i.e: Tweeter, Facebook and LinkedIn)

Functionalized MNP toolbox

MNPs with complementary chemistry to immobilize the engineered enzymes

AMF features to tune heat transfer V2.0/Public

Public final report on features that allow tuning MNPs heat transfer

Final round of project newsletters

Report on the preparation of the final round of Newsletters

Multienzyme hybrid composite toolbox V2.0_Public

Public report on final multienzyme hybrid composite toolbox simultaneously or sequentially activated under certain AMF settings

First round of project newsletters

Report on the preparation of the first round of Newsletters

Thermal nanoactuated synthesis of α-glucosyl-2-glycerate_Public

Public report on new AMFtuned process window for hightemperature synthesis of glucosyl2glycerate

Aminoacid cluster toolbox/Public

Public report on aminoacid cluster toolbox to allow enzyme orientation through target regions

Thermal nanoactuated sucrose conversion wo fructose decomposition_Public

Public report on new AMFtuned process window for hightemperature conversion of sucrose without fructose breakdown

His-tagged enzymes variants

His-tagged variants to immobilized enzyme through N- or C-terminus

Open Research Data Pilot (1)

Data Management Plan

Report on Data Management Plan

Publications

Peer reviewed articles (26)

Energy Transfer from Magnetic Iron Oxide Nanoparticles: Implications for Magnetic Hyperthermia

Auteurs: Gloria Tabacchi; Ilaria Armenia; Giovanni Bernardini; Norberto Masciocchi; Antonietta Guagliardi; Ettore Fois
Publié dans: ACS Appl. Nano Mater., Numéro 6-14, 2023, Page(s) 12914-12921, ISSN 2574-0970
Éditeur: American Chemical Society
DOI: 10.1021/acsanm.3c01643

Reproducibility and scalability of magnetic nanoheater synthesis

Auteurs: Ovejero, Jesus; Gallo-Cordova, Alvaro; Roca, Alejandro G.; Morales, M.P.; Veintimillas-Verdaguer, Sabino
Publié dans: Nanomaterials, Numéro 20794991, 2021, ISSN 2079-4991
Éditeur: MDPI AG
DOI: 10.3390/nano11082059

The Yin and Yang of epigenetics in the field of nanoparticles

Auteurs: Elettra Musolino; Christina Pagiatakis; Simone Serio; Marina Borgese; Federica Gamberoni; Rosalba Gornati; Giovanni Bernardini; Roberto Papait
Publié dans: Nanoscale Advances, Numéro 25160230, 2022, ISSN 2516-0230
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d1na00682g

Mixing iron oxide nanoparticles with different shape and size for tunable magneto-heating performance

Auteurs: Jesus G. Ovejero, Federico Spizzo, M. Puerto Morales, Lucia Del Bianco
Publié dans: Nanoscale, Numéro 13/11, 2021, Page(s) 5714-5729, ISSN 2040-3364
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0nr09121a

Remote Activation of Enzyme Nanohybrids for Cancer Prodrug Therapy Controlled by Magnetic Heating

Auteurs: Beatriz Torres-Herrero; Ilaria Armenia; Maria Alleva; Laura Asín; Sonali Correa; Cecilia Ortiz; Yilian Fernández-Afonso; Lucía Gutiérrez; Jesús M. de la Fuente; Lorena Betancor; Valeria Grazú
Publié dans: ACS Nano, Numéro 17-13, 2023, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.3c01599

Divalent Transition Metal Coatings for an Effective Protein-Nanoparticle Conjugation

Auteurs: Ilaria Armenia; Jesus G. Ovejero; Nicoll Zeballos Lema; Fernando López-Gallego; F. Javier Palomares; Sabino Veintemillas-Verdaguer; Jesús M. de la Fuente; M. Puerto Morales; Valeria Grazú
Publié dans: Applied Surface Science, Numéro 639, 2023, Page(s) Art. number 158221, ISSN 0169-4332
Éditeur: Elsevier BV
DOI: 10.2139/ssrn.4420984

How size, shape and assembly of magnetic nanoparticles give rise to different hyperthermia scenarios

Auteurs: Gavilan, H.; Simeonidis, K.; Myrovali, E.; Mazarío, E.; Chubykalo-Fesenko, O.; Chantrell, R.; Balcells, Ll.; Angelakeris, M; Morales, M.P.; Serantes, D.
Publié dans: Nanoscale, Numéro 20403364, 2021, ISSN 2040-3364
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d1nr03484g

Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles.

Auteurs: Raquel Moreno-Loshuertos; Ángel Millán; P. Tellez; Patricio Fernández-Silva; Luis Martínez-Lostao; Carlos D. S. Brites; Rafael Piñol; Albano N. Carneiro Neto; A. Martinez; Yuanyu Gu; Justyna Zeler; Justyna Zeler; Thiago Silva; Thiago Silva; Ana Isabel Gallego; Luís D. Carlos
Publié dans: Nano Letters, Numéro 3, 2020, ISSN 1530-6984
Éditeur: American Chemical Society
DOI: 10.1021/acs.nanolett.0c02163

Local Temperature Increments and Induced Cell Death in Intracellular Magnetic Hyperthermia

Auteurs: Yuanyu Gu; Rafael Piñol; Raquel Moreno-Loshuertos; Carlos D. S. Brites; Justyna Zeler; Abelardo Martínez; Guillaume Maurin-Pasturel; Patricio Fernández-Silva; Joaquín Marco-Brualla; Pedro Téllez; Rafael Cases; Rafael Navarro Belsué; Debora Bonvin; Luís D. Carlos; Angel Millán
Publié dans: ACS Nano, Numéro 17-7, 2023, Page(s) 6822-6832, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.3c00388

Tailoring the Magnetic and Structural Properties of Manganese/Zinc Doped Iron Oxide Nanoparticles through Microwaves-Assisted Polyol Synthesis

Auteurs: Porru, Margherita; Morales, Maria del Puerto; Gallo-Cordova, Alvaro; Espinosa, Ana; Moros, Maria; Brero, Francesca; Mariani, Manuel; Lascialfari, Alessandro; G. Ovejero, Jesus
Publié dans: Nanomaterials, Numéro 20794991, 2022, ISSN 2079-4991
Éditeur: MDPI
DOI: 10.3390/nano12193304

Magnetic hyperthermia with ε-Fe 2 O 3 nanoparticles

Auteurs: Yuanyu Gu, Marie Yoshikiyo, Asuka Namai, Debora Bonvin, Abelardo Martinez, Rafael Piñol, Pedro Téllez, Nuno J. O. Silva, Fredrik Ahrentorp, Christer Johansson, Joaquín Marco-Brualla, Raquel Moreno-Loshuertos, Patricio Fernández-Silva, Yuwen Cui, Shin-ichi Ohkoshi, Angel Millán
Publié dans: RSC Advances, Numéro 10/48, 2020, Page(s) 28786-28797, ISSN 2046-2069
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0ra04361c

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

Auteurs: Alejandro H. Orrego, Daniel Andrés-Sanz, Susana Velasco-Lozano, Mercedes Sanchez-Costa, José Berenguer, José M. Guisan, Javier Rocha-Martin, Fernando López-Gallego
Publié dans: Catalysis Science & Technology, 2021, ISSN 2044-4753
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d1cy00268f

Photonic and magnetic materials for on-demand local drug delivery

Auteurs: Armenia, Ilaria; Cuestas Ayllon, Carlos; Torres Herrero, Beatriz; Bussolari, Francesca; Alfranca, Gabriel; Grazú, Valeria; Martínez de la Fuente, Jesús
Publié dans: Advanced Drug Delivery Reviews, Numéro 0169409X, 2022, ISSN 0169-409X
Éditeur: Elsevier BV
DOI: 10.1016/j.addr.2022.114584

Antimicrobial Activity of Nanoconjugated Glycopeptide Antibiotics and Their Effect on Staphylococcus aureus Biofilm

Auteurs: Berini, Francesca; Orlandi, Viviana Teresa; Gamberoni, Federica; Martegani, Eleonora; Armenia, Ilaria; Gornati, Rosalba; Bernardini, Giovanni; Marinelli, Flavia
Publié dans: Frontiers in Microbiology, Numéro 1664302X, 2021, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2021.657431

Continuous production of magnetic iron oxide nanocrystals by oxidative precipitation

Auteurs: T. Asimakidou, A. Makridis, S. Veintemillas-Verdaguer, M.P. Morales, I. Kellartzis, M. Mitrakas, G. Vourlias, M. Angelakeris, K. Simeonidis
Publié dans: Chemical Engineering Journal, Numéro 393, 2020, Page(s) 124593, ISSN 1385-8947
Éditeur: Elsevier BV
DOI: 10.1016/j.cej.2020.124593

Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles

Auteurs: Patrizia Bonfanti, Anita Colombo, Melissa Saibene, Luisa Fiandra, Ilaria Armenia, Federica Gamberoni, Rosalba Gornati, Giovanni Bernardini, Paride Mantecca
Publié dans: Nanotoxicology, Numéro 14/2, 2020, Page(s) 196-213, ISSN 1743-5390
Éditeur: Informa Healthcare
DOI: 10.1080/17435390.2019.1685695

Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports

Auteurs: Sonali Correa, Magdalena Ripoll, Erienne Jackson, Valeria Grazú, Lorena Betancor
Publié dans: Catalysts, Numéro 10/6, 2020, Page(s) 669, ISSN 2073-4344
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal10060669

The Thermodynamics of Medial Vascular Calcification

Auteurs: Ángel Millán, Peter Lanzer, Víctor Sorribas
Publié dans: Frontiers in cell and developmental biology, 2021, ISSN 2296-634X
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fcell.2021.633465

Efficient Brownian oscillators and nanoheaters based on gallium-doped ε-Fe2O3

Auteurs: Yuanyu Gu, Nuno J. O. Silva, Marie Yoshikiyo, Asuka Namai, Rafael Piñol, Guillaume Maurin-Pasturel, Yuwen Cui, Shin-ichi Ohkoshi, Angel Millán ORCID, Abelardo Martínez
Publié dans: Chemical Communications, 2021, ISSN 1359-7345
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0cc07309a

High-Dose Exposure to Polymer-Coated Iron Oxide Nanoparticles Elicits Autophagy-Dependent Ferroptosis in Susceptible Cancer Cells

Auteurs: Thanpisit Lomphithak, Selin Helvacioglu, Ilaria Armenia, Sandeep Keshavan, Jesus g. Ovejero, Giovanni Baldi, Costanza Ravagli, Valeria Grazu, Bengt Fadeel
Publié dans: Nanomaterials, Numéro 13(11), 2023, Page(s) 1719, ISSN 2079-4991
Éditeur: MDPI
DOI: 10.3390/nano13111719

Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?

Auteurs: Berini, Francesca; Orlandi, Viviana; Gornati, Rosalba; Bernardini, Giovanni; Marinelli, Flavia
Publié dans: Biotechnology Advances, Numéro 07349750, 2022, ISSN 0734-9750
Éditeur: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107948

Nanoparticles for Magnetic Heating: When Two (or More) Is Better Than One

Auteurs: Jesus G. Ovejero; Federico Spizzo; M. Puerto Morales; Lucia Del Bianco
Publié dans: Materials, Numéro 20782489, 2021, ISSN 2078-2489
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ma14216416

Selective Magnetic Nanoheating: Combining Iron Oxide Nanoparticles for Multi-Hot-Spot Induction and Sequential Regulation.

Auteurs: Jesus G. Ovejero; Ilaria Armenia; David Serantes; Sabino Veintemillas-Verdaguer; Nicoll Zeballos; Fernando López-Gallego; Cordula Grüttner; Jesús M. de la Fuente; María del Puerto Morales; Valeria Grazú
Publié dans: Nano Letters, Numéro 15306984, 2021, ISSN 1530-6984
Éditeur: American Chemical Society
DOI: 10.1021/acs.nanolett.1c02178

Iron Oxide Nanoparticles with and without Cobalt Functionalization Provoke Changes in the Transcription Profile via Epigenetic Modulation of Enhancer Activity

Auteurs: Federica Gamberoni; Marina Borgese; Christina Pagiatakis; Ilaria Armenia; Valeria Grazù; Rosalba Gornati; Simone Serio; Roberto Papait; Giovanni Bernardini
Publié dans: Nano Letters, Numéro 23-19, 2023, Page(s) 9151-9159, ISSN 1530-6984
Éditeur: American Chemical Society
DOI: 10.1021/acs.nanolett.3c01967

Energetics of the Glycosyl Transfer Reactions of Sucrose Phosphorylase

Auteurs: Anisha Vyas, Bernd Nidetzky
Publié dans: Biochemistry, 2023, ISSN 0006-2960
Éditeur: American Chemical Society
DOI: 10.1021/acs.biochem.3c00080

Understanding MNPs Behaviour in Response to AMF in Biological Milieus and the Effects at the Cellular Level: Implications for a Rational Design That Drives Magnetic Hyperthermia Therapy toward Clinical Implementation.

Auteurs: David Egea-Benavente; Jesus G. Ovejero; Jesus G. Ovejero; María del Puerto Morales; Domingo F. Barber
Publié dans: Cancers, Vol 13, Iss 4583, p 4583 (2021), Numéro 20726694, 2021, ISSN 2072-6694
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13184583

Book chapters (2)

Thermal tuning of enzyme activity by magnetic heating

Auteurs: Ilaria Armenia, Francesca Bussolari, Manu Sanchez, Alvaro Gallo-Cordova, Jesus G. Ovejero, Eduardo Macedo de Melo, Federica GAmberoni, Marina Borgese, Simone Serio, J M. Guisan-Seijas, J. M. de la Fuente, Roberto Papait, Rosalba Gornati, Giovanni Bernardini, Nicolas Cassinelli, Doerte Rother, M. Puerto Morales, Fernando Lopez-Gallego, Valeria Grazu
Publié dans: Bionanocatalysis: from Design to Applications - Micro and Nano Technologies, 2023, Page(s) 117-159
Éditeur: Elsevier
DOI: 10.1016/b978-0-323-91760-5.00012-9

Magnetic Iron Oxide Colloids for Environmental Applications

Auteurs: Alvaro Gallo-Cordova, Daniela Almeida Streitwieser, María del Puerto Morales, Jesús G. Ovejero
Publié dans: Colloids - Types, Preparation and Applications [Working Title], 2021
Éditeur: IntechOpen
DOI: 10.5772/intechopen.95351