Development Of Microfluidic Based Low Cost Industrial Cryo-Ready Devices For Preservation Of Living Cells

Descripción del proyecto

Crioconservación basada en microfluidos de células vivas y biomuestras

El aumento de la demanda de productos de terapia celular conlleva la necesidad de que la Organización Mundial de la Salud emita nuevas normativas para el almacenamiento clínico y las aplicaciones de estos productos desarrollados. Una necesidad inmediata es la creación de investigaciones e infraestructuras para el almacenamiento prolongado de estos productos. Los procesos actuales, como la crioconservación, la liofilización y la desecación, son altamente tóxicos para la viabilidad celular. El proyecto financiado con fondos europeos MCRD propone una colaboración con la empresa francesa Eden Tech para desarrollar un dispositivo listo para la criogenización a base de microfluidos para el almacenamiento inmediato y la realización de ensayos con muestras biológicas o de tipos celulares. El nuevo producto preparará el terreno para nuevos estudios avanzados de biología física.

Objetivo

Innovation in development of cell therapy products are growing rapidly. As per the report of WHO, in the year 2015, total 38 such innovation has been taken place in EU region and other developed countries. Such accelerated growth of the technology leads WHO to configure regulatory issues for the clinical storage and applications of these developed products. Thus, on the basis of current requirements, there is a immediate need to develop systemic research and infrastructure for storage of the developed biological products for longer duration of time. Cryopreservation, lyophilization, and desiccation are the major techniques been used for the preservation of biological products, namely different types of cells. But all above mentioned process are highly toxic while considering cellular viability. At the same time, this processes are bulky and involves substantial cost affair.
In current project proposal, ER in collaboration with Eden Microfluidics have proposed development of a microfluidic based cryo-ready device for plug-and-play type cell / biological sample storage, testing, and laboratory usage. The proposed technique is highly inter-disciplinary, and with the experience of ER and his mentors from Eden Microfluidics, they are confident for the development of the product. The development of the product will yield new insight of physical biology in the field of magneto-hydrodynamics, biological thermodynamics, and bio-microfluidics.
The proposed project is not only limited to the development of product, but it will extend the work for marketing and production of the product, and dissemination of the acquired field of science to the national and international universities as text contents (in form of book chapters), laboratory tutorials through the collaborations of ER. The developed cryo-ready microfluidic device will also foster ER for involve in EU region as a start-up, and thus contribute to the economical growth of the region directly.