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Visualising how proteins fold DNA into topologically associating domains in single human cells

Project description

3D ultrastructure of DNA organisation in single cells

Understanding how genetic information is regulated inside the cell represents an intriguing challenge of modern biology. Genome-wide studies have revealed a hierarchical genome organisation by structuring proteins, which regulates fundamental processes such as transcription, replication and DNA repair. Topologically associating domains (TADs) are functional building blocks of chromatin organisation above the nucleosome level. The now available super-resolution microscopy set-ups allow the study of genomic structures and their dynamics in situ at a resolution of 10 nm, which is comparable to the size of a nucleosome. The aim of the EU-funded ChromaSTORM project is to reveal the principles of TAD organisation in human cells using an imaging-based approach to visualize its genomic component as well as the architectural proteins. The project will provide the first 3D description of this central chromatin super-structure.

Coordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Net EU contribution
€ 174 806,40
Address
Meyerhofstrasse 1
69117 Heidelberg
Germany

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Region
Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis
Activity type
Research Organisations
Other funding
€ 0,00