Project description DEENESFRITPL Improved light-sheet microscopy prototype enables a better view of cell dynamics Light-sheet fluorescence microscopy (LSFM) is a technique in which a very thin slice of a sample is illuminated by a laser source perpendicularly to the direction of observation. The EU-funded MAFIn project developed a universally compatible module capable of fast volumetric imaging that combines LSFM with waveform coding. The latter introduces a phase mask in the detection arm of the LSFM platform to extend the depth of field. A deconvolution operation needs to be applied as the phase mask inevitably distorts the obtained images. The project provided a calibration procedure with optimised deconvolution strategies so that LSFM with wavefront coding can be performed in a simple way. The new prototype holds great promise for higher-resolution imaging of cell dynamics. Show the project objective Hide the project objective Objective The aim of this proposal is to evolve the current set-up used in the FET Open project MESOBRAIN to a functional prototype capable of running the full hardware and any related software, in an easy, stable and modular way, which can be operated by an inexperienced user. The current set-up is capable of fast volumetric imaging under high resolution (x, y, z) conditions and is based on the combination of a light sheet fluorescence microscope (LSFM) with Wavefront coding (WFC) techniques. This allows obtaining high resolution images at a speed approaching 100Volumes/sec. Beyond the WFC capabilities, the new MAFIn module will be based on the implementation of adaptive optics (AO) strategies for aberration compensations. Therefore, the module will be also able to correct for the aberration introduced by the system and by the sample itself. As a result, this module for aberration correction and fast volumetric imaging will be universal, as it will be able to be adapted to any existing LSFM, either to current commercial systems or to custom-made setups in research labs, and easy to use by non-experts in life sciences applications. This unique MAFIn prototype will be able to observe the fast cellular and intracellular dynamics that occur in biological samples in 3D with high resolutions. Therefore, MAFIn will significantly expand the impact of the FET Open project MESOBRAIN. Fields of science natural sciencesphysical sciencesopticsmicroscopy Programme(s) H2020-EU.1.2. - EXCELLENT SCIENCE - Future and Emerging Technologies (FET) Main Programme H2020-EU.1.2.1. - FET Open Topic(s) FETOPEN-03-2018-2019-2020 - FET Innovation Launchpad Call for proposal H2020-FETOPEN-2018-2020 See other projects for this call Sub call H2020-FETOPEN-2018-2019-2020-03 Funding Scheme CSA - Coordination and support action Coordinator FUNDACIO INSTITUT DE CIENCIES FOTONIQUES Net EU contribution € 100 000,00 Address AVINGUDA CARL FRIEDRICH GAUSS 3 08860 Castelldefels Spain See on map Region Este Cataluña Barcelona Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 100 000,00
