Project description
Novel integrated serological surveillance of malaria and neglected tropical diseases
Malaria and neglected tropical diseases (NTDs) like lymphatic filariasis, onchocerciasis, trachoma and schistosomiasis affect 2 billion people yearly. Successfully targeting these diseases calls for integrated surveillance strategies. Malaria surveillance is routinely implemented through cross-sectional surveys; this provides as an opportunity to introduce serological testing by measuring antibodies to multiple pathogens in blood samples collected for malaria surveillance. The goal of the EU-funded MultiSeroSurv project is to characterise the sero-epidemiology of malaria and NTDs in longitudinal cohort studies in Ethiopia, Cambodia, Papua New Guinea and Senegal, measuring antibodies to 34 antigens from 12 pathogens in the single blood samples. The collected data will provide validation for the use of serology in detecting infections and determining how concurrent and past exposures to multiple parasites can be estimated through multiplex data analysis from cross-sectional surveys.
Objective
Malaria and neglected tropical diseases (NTDs) such as lymphatic filariasis, onchocerciasis, trachoma and schistosomiasis affect almost 2 billion people every year. Coordinated targeting of these diseases will aid future control and elimination campaigns, but this will require integrated surveillance strategies. Malaria surveillance is routinely implemented through cross-sectional surveys where blood samples are tested for parasites. A major barrier to routine NTD surveillance is the range of samples required for parasite diagnosis: stool, urine, blood, eye swabs and skin snips. Instead of direct detection of parasites, there is an opportunity to implement serological surveillance by measuring antibodies to multiple pathogens in blood samples collected for malaria surveillance.
This proposal has four aims: (i) Characterise the sero-epidemiology of malaria and NTDs in longitudinal cohort studies in Senegal, Ethiopia, Cambodia and Papua New Guinea; (ii) Measure antibodies to 34 antigens from 12 pathogens from single blood samples; (iii) Model antibody kinetics and validate the use of serology for detecting infections; and (iv) Demonstrate how a population’s concurrent and past exposure to multiple parasites can be estimated by analysing multiplex data from cross-sectional surveys.
These aims will be achieved through innovative epidemiological studies, new technologies, and especially developed analytic methods, including: (1) utilisation of multiplex bead-based Luminex assays; (2) mixed-effects models in a Bayesian framework with data augmentation to identify serologically suspected infections, with validation against confirmed infections; and (3) statistical algorithms for reconstructing long-term and recent transmission trends. This interdisciplinary project will undertake fundamental research in analytic methods for processing complex multiplex data and converting it to the actionable information needed for integrated serological surveillance of malaria and NTDs.
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Funding Scheme
ERC-STG - Starting GrantHost institution
75724 Paris
France