Periodic Reporting for period 2 - MultiSeroSurv (Algorithms and multiplex assays for integrated serological surveillance of malaria and neglected tropical diseases)
Período documentado: 2021-08-01 hasta 2023-01-31
This proposal has four aims: (i) Characterise the sero-epidemiology of malaria and NTDs in longitudinal cohort studies in Senegal, Ethiopia, Cambodia and Papua New Guinea; (ii) Measure antibodies to 34 antigens from 12 pathogens from single blood samples; (iii) Model antibody kinetics and validate the use of serology for detecting infections; and (iv) Demonstrate how a population’s concurrent and past exposure to multiple parasites can be estimated by analysing multiplex data from cross-sectional surveys.
These aims will be achieved through innovative epidemiological studies, new technologies, and especially developed analytic methods, including: (1) utilisation of multiplex bead-based Luminex assays; (2) mixed-effects models in a Bayesian framework with data augmentation to identify serologically suspected infections, with validation against confirmed infections; and (3) statistical algorithms for reconstructing long-term and recent transmission trends.
This interdisciplinary project will undertake fundamental research in analytic methods for processing complex multiplex data and converting it to the actionable information needed for integrated serological surveillance of malaria and NTDs.
(1) Development of high-throughput serological multi-pathogen assays using multiplex Luminex technology (Objective 2)
(2) Development of non-linear mixed-effects models of antibody kinetics (Objective 3)