Project description
The next generation of 'next generation' sequencing methods is on the horizon
The sequence of nucleotides in DNA reveals information about genes, chromosomes and even an entire genome. High-throughput 'next generation' sequencing methods have now made it possible to sequence the human genome in a day. Contributing to the meteoric increase in our understanding of genetics and mutations in health and disease, the techniques face an important limitation. EU-funded scientists are addressing it, significantly enhancing the resolution of both spatial localisation and organisation of sequences within single cells in intact tissues. Combined with faster and more detailed analyses and a decrease in cost, the technology could spur a new age of discovery.
Objective
Next generation sequencing has led to revolutionary discoveries in the fields of genetics, genomics, epigenetics and transcriptional regulation. The main limitation of this technology relies on the loss of spatial information: NGS is unable to retrieve the organization of nucleic acids (DNA/RNA) in the cell or within the more complex structure of the tissue. We have recently developed a novel imaging-based technology, Hi-M, that enables the simultaneous detection of tens of DNA and RNA species in single cells within the context of intact tissues. In NGI, we propose to make major improvements to drastically reduce data acquisition and analysis time, considerably increase throughput and number of independent nucleic-acid species detected, and improve the robustness of acquisition and the user-friendliness of analysis. These objectives will be achieved by implementing, testing and validating a novel combinatorial labeling scheme, parallelized acquisition, improvement of the liquid-handling robot to handle hundreds of independent species, and on-the-fly analysis using deep learning technologies to provide more robust data analysis pipelines and adapt acquisition parameters in real time. These improvements will enable simultaneous detection of thousands of species (DNA, RNA or protein) in single-cells with spatial resolution within the context of complex organisms and tissues. Because of these important advantages over existing technologies, NGI will be key to future discoveries in the fields of genetics, genomics and transcription. Critically, NGI will also have a large impact in other fundamental and applied fields where knowledge of spatial organization of transcription and 3D chromosome organization at the single-cell level are relevant: neuroscience and neurological diseases, diabetes, cancer, etc. Thus, NGI has the potential to become an ubiquitous tool not only in academic science but also at the clinic.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences data science
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences genetics DNA
- natural sciences computer and information sciences artificial intelligence machine learning deep learning
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-POC-LS - ERC Proof of Concept Lump Sum Pilot
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2019-PoC
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75794 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.