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Barcoding gene expression dynamics at single-molecule resolution

Project description

Gene expression at single-molecule resolution

The EU-funded DYNOME project will study gene expression kinetics in individual cells at single-molecule resolution, for better understanding of how biological variability arises from the laws of statistics. The research combines several innovative approaches: a super-resolution detect-and-bleach strategy, multi-colour barcoding to monitor up to six genes in parallel, lineage tracking tools, and lab-on-a-chip bioreactors to control growth conditions. This biodynamics platform will allow monitoring of the gene expression dynamics at single-molecule level in single cells at the same time for several genes over many generations. As a model system, DYNOME will use stochastic decision-making events in bacteria and eukaryotic cells, involving a stochastic phenotype switch (Bacillus subtilis), development of non-genetic drug resistance (Escherichia coli), and cell cycle control (Saccharomyces cerevisiae). Deciphering cell individuality with kinetic models will be a breakthrough for development of drugs against bacterial invasions, for design of new functionalities in cells, and for the general concept of biological variabilities.

Host institution

WEIZMANN INSTITUTE OF SCIENCE
Net EU contribution
€ 2 368 531,00
Address
Herzl Street 234
7610001 Rehovot
Israel

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Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00

Beneficiaries (1)

WEIZMANN INSTITUTE OF SCIENCE
Israel
Net EU contribution
€ 2 368 531,00
Address
Herzl Street 234
7610001 Rehovot

See on map

Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00