Project description
Gene expression at single-molecule resolution
The EU-funded DYNOME project will study gene expression kinetics in individual cells at single-molecule resolution, for better understanding of how biological variability arises from the laws of statistics. The research combines several innovative approaches: a super-resolution detect-and-bleach strategy, multi-colour barcoding to monitor up to six genes in parallel, lineage tracking tools, and lab-on-a-chip bioreactors to control growth conditions. This biodynamics platform will allow monitoring of the gene expression dynamics at single-molecule level in single cells at the same time for several genes over many generations. As a model system, DYNOME will use stochastic decision-making events in bacteria and eukaryotic cells, involving a stochastic phenotype switch (Bacillus subtilis), development of non-genetic drug resistance (Escherichia coli), and cell cycle control (Saccharomyces cerevisiae). Deciphering cell individuality with kinetic models will be a breakthrough for development of drugs against bacterial invasions, for design of new functionalities in cells, and for the general concept of biological variabilities.
Fields of science
- natural sciencesbiological sciencesmicrobiologybacteriology
- engineering and technologyother engineering and technologiesmicrotechnologylab on a chip
- natural sciencesphysical sciencesopticsmicroscopysuper resolution microscopy
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistance
Programme(s)
Funding Scheme
ERC-COG - Consolidator Grant
Host institution
7610001 Rehovot
Israel
See on map
Beneficiaries (1)
7610001 Rehovot
See on map