Periodic Reporting for period 1 - CAPTUR3D (CAPTURING THE PHYSICS OF LIFE ON 3D-TRAFFICKING SUBCELLULAR NANOSYSTEMS)
Reporting period: 2021-03-01 to 2022-08-31
In line with the project DoA (and related Gantt diagram) the first 18 months since project start encompass the activities of WP0, WP1, WP2 and, marginally, WP3. Concerning the Preparatory Phase of WP0, all the planned tasks were successfully executed. In brief, task 0.A and 0.B (WP0-M1: islets imaging) were successfully accomplished and resulted in 2 publications (1 full article and 1 conference proceedings, both in press). Task 0.C (WP0-M2: Cell-based characterization of average granule structural and dynamic properties) was accomplished and results were included in 2 publications (Ferri et al. JoVE 2021; Ferri et al. Pharmaceutics 2021). Task 0.D (WP0-M3: Single-ISG feedback-based tracking and imaging) was accomplished and results were included in 1 publication (Begarani et al. IJMS 2022). The potential of the strategy proposed was also discussed in 1 review article (Ferri et al. IJMS 2021). The successful completion of the preparatory phase laid the ground to move to the production phase (WP1-2-3). Concerning WP1, task 1.A (WP1-M1: Single-granule trajectories at millisecond temporal resolution) was accomplished within the first 18 months as planned and results are included in 1 publication (Begarani et al. IJMS 2022). Experiments on Task 1.B and 1.C are ongoing. As planned, the tasks of WP2 and WP3 were also activated in this reporting period. Overall, the results obtained so far will be presente in 2 conferences (1 national, SID2022, and 1 international, EASD2022) by means of an oral presentation by the PI and two posters by members of the Team.
Overall, the project development follows the proposed plan, with a few adjustments which are further explained below.
Overall, the project development follows the proposed plan, with a few adjustments which are further explained below.