CAPTURING THE PHYSICS OF LIFE ON 3D-TRAFFICKING SUBCELLULAR NANOSYSTEMS

Project Information

CAPTUR3D

Grant agreement ID: 866127

DOI

10.3030/866127

EC signature date 16 March 2020

Start date 1 March 2021

End date 28 February 2026

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 985 750,00

EU contribution

€ 1 985 750,00

Coordinated by

SCUOLA NORMALE SUPERIORE
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (14)

Optical Nanoscopy of Cytokine-Induced Structural Alteration of Endoplasmic Reticulum and Golgi Apparatus in Insulin Secreting Cells

Author(s): Licia Anna Pugliese, Valentina De Lorenzi, Marta Tesi, Piero Marchetti, Francesco Cardarelli
Published in: International Journal of Molecular Sciences, 2024, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.20944/preprints202408.1000.v1

Mechanistic Insights into the Superior DNA Delivery Efficiency of Multicomponent Lipid Nanoparticles: An In Vitro and In Vivo Study

Author(s): Erica Quagliarini; Junbiao Wang; Serena Renzi; Lishan Cui; Luca Digiacomo; Gianmarco Ferri; Luca Pesce; Valentina De Lorenzi; Giulia Matteoli; Heinz Amenitsch; Laura Masuelli; Roberto Bei; Daniela Pozzi; Augusto Amici; Francesco Cardarelli; Cristina Marchini; Giulio Caracciolo
Published in: ACS Applied Materials and Interfaces, 2022, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.2c20019

Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor

Author(s): Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Giulia Matteoli, Gianmarco Ferri, Barbara Storti, Ranieri Bizzarri, Francesco Cardarelli
Published in: Communications Biology, Issue 8, 2025, ISSN 2399-3642
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-07758-w

β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications

Author(s): Gianmarco Ferri; Luca Pesce; Marta Tesi; Piero Marchetti; Francesco Cardarelli
Published in: International Journal of Molecular Sciences, Issue 14220067, 2021, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222312820

Pioglitazone Phases and Metabolic Effects in Nanoparticle-Treated Cells Analyzed via Rapid Visualization of FLIM Images

Author(s): Biagio Todaro, Luca Pesce, Francesco Cardarelli, Stefano Luin
Published in: Molecules, Issue 29, 2025, Page(s) 2137, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules29092137

Unveiling nanoscale optical signatures of cytokine‑induced β‑cell dysfunction

Author(s): Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Luca Pesce, Francesco Cardarelli
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-40272-9

Pair correlation microscopy of intracellular molecular transport

Author(s): Julissa Sanchez-Velasquez, Ashleigh Solano, Michelle A. Digman, Enrico Gratton, Francesco Cardarelli, Elizabeth Hinde
Published in: Nature Protocols, Issue 20, 2025, Page(s) 1651-1677, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-024-01097-6

Fluorescence Lifetime Nanoscopy of Liposomal Irinotecan Onivyde: From Manufacturing to Intracellular Processing

Author(s): Mario Bernardi, Giovanni Signore, Aldo Moscardini, Licia Anna Pugliese, Luca Pesce, Fabio Beltram, Francesco Cardarelli
Published in: ACS Applied Bio Materials, 2023, ISSN 2576-6422
Publisher: American Chemical Society
DOI: 10.1021/acsabm.3c00478

Single-cell imaging of α and β cell metabolic response to glucose in living human Langerhans islets

Author(s): Fabio Azzarello, Luca Pesce, Valentina De Lorenzi, Gianmarco Ferri, Marta Tesi, Silvia Del Guerra, Piero Marchetti, Francesco Cardarelli
Published in: Communications Biology, 2022, ISSN 2399-3642
Publisher: Nature Publishing Group
DOI: 10.1038/s42003-022-04215-w

Spatiotemporal Correlation Spectroscopy Reveals a Protective Effect of Peptide-Based GLP-1 Receptor Agonism against Lipotoxicity on Insulin Granule Dynamics in Primary Human β-Cells.

Author(s): Gianmarco Ferri; Marta Tesi; Luca Pesce; Marco Bugliani; Francesca Grano; Margherita Occhipinti; Mara Suleiman; Carmela De Luca; Lorella Marselli; Piero Marchetti; Francesco Cardarelli
Published in: Pharmaceutics, Issue 19994923, 2021, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13091403

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Author(s): Gianmarco Ferri, Fabio Azzarello, Francesca D'Autilia, Francesco Cardarelli
Published in: Journal of Visualized Experiments, Issue 1940087X, 2021, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/62790

Investigating the mechanism of action of DNA-loaded PEGylatedlipid nanoparticles

Author(s): Luca Digiacomo, Serena Renzi, Erica Quagliarini, Daniela Pozzi, Heinz Amenitsch, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Francesco Cardarelli, Giulio Caracciolo
Published in: Nanomedicine: Nanotechnology, Biology, and Medicine, 2023, ISSN 1549-9634
Publisher: Elsevier BV
DOI: 10.1016/j.nano.2023.102697

Measuring molecular diffusion in dynamic subcellular nanostructures by fast Raster Image Correlation Spectroscopy and 3D orbital tracking

Author(s): Filippo Begarani; Francesca D’Autilia; Gianmarco Ferri; Luca Pesce; Fabio Azzarello; Valentina De Lorenzi; William Durso; Ambra Del Grosso; Marco Cecchini; Francesco Cardarelli
Published in: International Journal of Molecular Sciences, Issue 14220067, 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23147623

Machine-learning-guided recognition of α and β cells from label-free infrared micrographs of living human islets of Langerhans

Author(s): Fabio Azzarello, Francesco Carli, Valentina De Lorenzi, Marta Tesi, Piero Marchetti, Fabio Beltram, Francesco Raimondi, Francesco Cardarelli
Published in: Scientific Reports, Issue 14, 2024, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-024-65161-7

Other (3)

Measuring pH in insulin secretory granules using phasor fluorescence lifetime imaging of a genetically encoded sensor

Author(s): Francesco Cardarelli, Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Giulia Matteoli, Barbara Storti, Ranieri Bizzarri
Published in: Research Square, 2025
Publisher: Springer Science and Business Media LLC
DOI: 10.21203/rs.3.rs-4968583/v1

Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks

Author(s): Mario Bernardi, Francesco Cardarelli
Published in: Biorxiv, 2023
Publisher: Cold Spring Harbor
DOI: 10.1101/2023.08.30.555392

Direct optical nanoscopy unveils signatures of cytokine-induced β-cell structural and functional stress

Author(s): Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Francesco Cardarelli, Luca Pesce
Published in: BiorXiv, 2023
Publisher: Cold Spring Harbor
DOI: 10.1101/2023.02.20.529190

Conference proceedings (4)

Single-cell-resolution imaging of alpha/beta cell metabolic response to glucose stimulation in living human-derived Langerhans islets

Author(s): Francesco Cardarelli; Fabio Azzarello; Luca Pesce; Valentina De Lorenzi; Gianmarco Ferri; Marta Tesi; Silvia Del Guerra; Piero Marchetti
Published in: Issue 3, 2022
Publisher: Springer

Single-cell-resolution imaging of alpha/beta cell metabolic response to glucose stimulation in living human-derived Langerhans islets

Author(s): Francesco Cardarelli, Fabio Azzarello, Luca Pesce, Valentina De Lorenzi, Gianmarco Ferri, Marta Tesi, Silvia Del Guerra, Piero Marchetti
Published in: DIABETOLOGIA - ISSN: 1432-0428, 2022, Page(s) S18-S19
Publisher: SPRINGER
DOI: 10.1007/s00125-022-05755-w

15th EBSA congress, June 30 – July 4, 2025, Rome, Italy – Abstracts

Author(s): Samuele Ghignoli; Valentina De Lorenzi; Gianmarco Ferri; Ranieri Bizzarri; Francesco Cardarelli
Published in: European Biophysics Journal, Issue 54, 2025, Page(s) 1-273, ISSN 0175-7571
Publisher: Springer Verlag
DOI: 10.1007/s00249-025-01757-9

Direct optical nanoscopy unveils signatures of cytokine-induced β-cell structural and functional stress

Author(s): Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Luca Pesce, Francesco Cardarelli
Published in: DIABETOLOGIA - ISSN: 1432-0428, 2023
Publisher: Springer
DOI: 10.1007/s00125-023-05969-6