Project description DEENESFRITPL Novel technology for cell migration and invasion analyses Cell interactions with the immediate microenvironment define many cellular functions such as spreading, adhesion, migration and differentiation. Cell migration is essential for homeostasis, wound healing, inflammation, tumor progression and metastases formation. The development of highly accurate methods to study cellular migration and transmigration is important for the understanding of fundamental biological processes and for cell-based therapy and drug screening. The EU-funded TOPOCELL project proposes the development of a microfabricated substrate to control cell confinement and topography, creating a platform to analyse cellular responses. The project designed elastomeric substrates that contain ordered micron-sized pillars allowing cells to transmigrate through versatile confined spaces. The versatility of the method is expected to lead to new opportunities for fundamental research in cell biology. Show the project objective Hide the project objective Objective Cell migration is essential for tissue development, homeostasis, tumor progression, including the responses to wounds and inflammation. Cell interactions with their microenvironment affect many cellular functions such as spreading, migration and even differentiation. These interactions can be studied by incorporating micro- and nanotechnology-related tools. The design of substrates based on these technologies offers new possibilities to probe the cellular responses to changes in their physical environment. The investigations of the physical interactions of cells and their surrounding matrix can be carried out in well-defined and near physiological conditions. In tissues, cells encounter confined environments and narrow spaces that could favor or prevent migration. In the DURACELL ERC project, we are studying the impact of substrate stiffness on cell migration. We propose to develop and use microfabricated substrates to control substrate confinement and topography and thus analyze cellular responses. Such elastomeric substrates are designed that contain ordered micron-sized pillars allowing cells to transmigrate through versatile confined spaces. The development of accurate methods to study cellular transmigration is important to answer fundamental biological processes and for cell-based therapy and drug screening. The well-accepted methods for transmigration assays including Boyden chambers, microfluidic devices present important limitations including spatial and temporal resolutions, limited variability and control of porosity composition and geometry. To overcome these limitations, the goal of TRANSCELL is to develop in-plane micropillar substrates whose geometry can be easily tuned to control and analyze cell transmigration, cell sorting and ultimately genetic expression profiles. We anticipate that the versatility of this method will offer new opportunities for fundamental research in cell biology, but also in cell therapies and drug screening. Fields of science natural sciencesphysical sciencesastronomyplanetary sciencesplanetary geologynatural sciencesbiological sciencescell biologynatural sciencesmathematicspure mathematicsgeometrymedical and health sciencesmedical biotechnologycells technologiesmedical and health sciencesbasic medicinephysiologyhomeostasis Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-2019-POC - ERC Proof of Concept Grant Call for proposal ERC-2019-PoC See other projects for this call Funding Scheme ERC-POC-LS - ERC Proof of Concept Lump Sum Pilot Coordinator CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Net EU contribution € 142 100,00 Address Rue michel ange 3 75794 Paris France See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window EU contribution No data Beneficiaries (2) Sort alphabetically Sort by Net EU contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS France Net EU contribution € 142 100,00 Address Rue michel ange 3 75794 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window EU contribution No data Third-party Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure. CNRS INNOVATION France Net EU contribution € 7 900,00 Address 79 avenue edouard vaillant le quintet bat d 92100 Boulogne-billancourt See on map Region Ile-de-France Ile-de-France Hauts-de-Seine Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window EU contribution No data
