Designer enzymes featuring unnatural amino acids as catalytic residue

Project Information

DENZUAC

Grant agreement ID: 885396

DOI

10.3030/885396

Project closed

EC signature date 21 April 2020

Start date 1 September 2020

End date 31 August 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 499 996,00

EU contribution

€ 2 499 996,00

Coordinated by

RIJKSUNIVERSITEIT GRONINGEN
Netherlands

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (16)

Artificial Gold Enzymes Using a Genetically Encoded Thiophenol‐Based Noble‐Metal‐Binding Ligand

Author(s): Mathijs J. Veen, Friso S. Aalbers, Henriëtte J. Rozeboom, Andy‐Mark W. H. Thunnissen, Daniel F. Sauer, Gerard Roelfes
Published in: Angewandte Chemie International Edition, Issue 64, 2025, Page(s) e202421912, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202421912

Unlocking Iminium Catalysis in Artificial Enzymes to Create a Friedel-Crafts Alkylase

Author(s): Reuben B. Leveson-Gower; Zhi Zhou; Ivana Drienovská; Gerard Roelfes
Published in: ACS Catalysis, Issue 3, 2021, Page(s) 6763–6770, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c00996

Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy.

Author(s): Aditya Iyer; Maxim Baranov; Alexander J. Foster; Shreyans Chordia; Gerard Roelfes; Rifka Vlijm; Geert van den Bogaart; Bert Poolman
Published in: Acs Chemical Biology, 16, 891 - 904, Issue 5, 2021, Page(s) 891–904, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.1c00100

Selective Aza‐Michael Addition to Dehydrated Amino Acids in Natural Antimicrobial Peptides

Author(s): Michela Vargiu, Yanli Xu, Oscar P. Kuipers, Gerard Roelfes
Published in: ChemBioChem, Issue 25, 2024, Page(s) e202400043, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202400043

Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis

Author(s): Bart Brouwer, Franco Della-Felice, Jan Hendrik Illies, Emilia Iglesias-Moncayo, Gerard Roelfes, Ivana Drienovská
Published in: Chemical Reviews, Issue 124, 2024, Page(s) 10877-10923, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.4c00136

Biocatalytic Friedel-Crafts Reactions

Author(s): Reuben B. Leveson‐Gower; Gerard Roelfes
Published in: ChemCatChem, Issue 1, 2022, Page(s) e202200636, ISSN 1867-3899
Publisher: Wiley-VCH GmbH
DOI: 10.1002/cctc.202200636

Tandem Friedel-Crafts-Alkylation-Enantioselective-Protonation by Artificial Enzyme Iminium Catalysis

Author(s): Reuben B. Leveson-Gower; Ruben de Boer; Gerard Roelfes
Published in: ChemCatChem, Issue 1, 2022, Page(s) e202101875, ISSN 1867-3899
Publisher: Wiley-VCH GmbH
DOI: 10.1002/cctc.202101875

Genetically encoded 3-aminotyrosine as catalytic residue in a designer Friedel–Crafts alkylase

Author(s): Bart Brouwer, Franco Della-Felice, Andy-Mark W. H. Thunnissen, Gerard Roelfes
Published in: Chemical Science, Issue 16, 2025, Page(s) 8721-8728, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d5sc01055a

Repurposed and artificial heme enzymes for cyclopropanation reactions.

Author(s): Gerard Roelfes
Published in: Journal of Inorganic Biochemistry, 222:111523. ELSEVIER SCIENCE INC, Issue 4, 2021, Page(s) 111523, ISSN 0162-0134
Publisher: Elsevier BV
DOI: 10.1016/j.jinorgbio.2021.111523

Boron Designer Enzyme with a Hybrid Catalytic Dyad

Author(s): Lars Longwitz, Marijn D. Kamer, Bart Brouwer, Andy-Mark W. H. Thunnissen, Gerard Roelfes
Published in: ACS Catalysis, Issue 14, 2024, Page(s) 18469-18476, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.4c06052

Boron catalysis in a designer enzyme

Author(s): Lars Longwitz, Reuben B. Leveson-Gower, Henriëtte J. Rozeboom, Andy-Mark W. H. Thunnissen, Gerard Roelfes
Published in: Nature, Issue 629, 2024, Page(s) 824-829, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-024-07391-3

Computation-guided engineering of distal mutations in an artificial enzyme

Author(s): Fabrizio Casilli, Miquel Canyelles-Niño, Gerard Roelfes, Lur Alonso-Cotchico
Published in: Faraday Discussions, Issue 252, 2024, Page(s) 262-278, ISSN 1359-6640
Publisher: Royal Society of Chemistry
DOI: 10.1039/d4fd00069b

Synergistic Catalysis of Tandem Michael Addition/Enantioselective Protonation Reactions by an Artificial Enzyme.

Author(s): Zhi Zhou; Gerard Roelfes
Published in: ACS Catalysis, Issue 3, 2021, Page(s) 366–9369, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c02298

An Artificial Copper‐Michaelase Featuring a Genetically Encoded Bipyridine Ligand for Asymmetric Additions to Nitroalkenes

Author(s): Ru Jiang, Fabrizio Casilli, Andy‐Mark W. H. Thunnissen, Gerard Roelfes
Published in: Angewandte Chemie International Edition, Issue 64, 2025, Page(s) e202423182, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202423182

An In Vivo Biocatalytic Cascade Featuring an Artificial Enzyme Catalyzed New-to-Nature Reaction

Author(s): Linda Ofori Atta; Zhi Zhou; Gerard Roelfes
Published in: Angewandte Chemie International Edition, Issue 2, 2023, Page(s) e202214191, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202214191

Evolutionary Specialization of a Promiscuous Designer Enzyme

Author(s): Reuben B. Leveson-Gower, Laura Tiessler-Sala, Henriette J. Rozeboom, Andy-Mark W. H. Thunnissen, Jean-Didier Maréchal, Gerard Roelfes
Published in: ACS Catalysis, Issue 15, 2025, Page(s) 1544-1552, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.4c06409

Non-peer reviewed articles (1)

Microbial cell factories for cycloalkene synthesis

Author(s): Ru Jiang, Gerard Roelfes
Published in: Nature Synthesis, Issue 3, 2024, Page(s) 1070-1071, ISSN 2731-0582
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s44160-024-00584-8