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Three-photon fluorescence imaging deep inside scattering media

Project description

Shedding new light on optical microscopy

At the intersection between molecular biology and in vivo imaging is molecular imaging. Progress in technology during recent decades has leveraged the development of high-resolution microscopy. Light microscopy – invented more than 400 years ago – is among the least invasive techniques used to access information from biological scales in living cells. The EU-funded DEEP3P project will address one of the main limitations of optical microscopy: light scattering confines it to the outermost tissue layers. The aim of the project is to establish scattering correction through wavefront shaping in higher-order multiphoton microscopy (three-photon fluorescence imaging) to surpass the scattering barrier and reach unprecedented depths in optical microscopy.

Objective

Investigating the complex structure and functioning of biological systems through light microscopy represents a foundational pillar of the life sciences. However, the very complexity of the samples cellular structure leads to one of the main limitations of optical microscopy: light scattering confines it to the outer most tissue layers. In the past decades, multiphoton microscopy successfully extended the accessible depth ranges and on a different front, advances in optical wavefront shaping showed that scattering can be compensated for, even in regimes where light fully lost its initial directionality. The aim of this action is to establish scattering correction through wavefront shaping in higher-order multiphoton microscopy, namely three-photon fluorescence imaging, to surpass the scattering barrier and reach unprecedented depths in optical microscopy.

Fields of science (EuroSciVoc)

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2019

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Coordinator

SORBONNE UNIVERSITE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 184 707,84
Address
21 RUE DE L'ECOLE DE MEDECINE
75006 PARIS
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 184 707,84
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