Project description
Shedding new light on optical microscopy
At the intersection between molecular biology and in vivo imaging is molecular imaging. Progress in technology during recent decades has leveraged the development of high-resolution microscopy. Light microscopy – invented more than 400 years ago – is among the least invasive techniques used to access information from biological scales in living cells. The EU-funded DEEP3P project will address one of the main limitations of optical microscopy: light scattering confines it to the outermost tissue layers. The aim of the project is to establish scattering correction through wavefront shaping in higher-order multiphoton microscopy (three-photon fluorescence imaging) to surpass the scattering barrier and reach unprecedented depths in optical microscopy.
Objective
Investigating the complex structure and functioning of biological systems through light microscopy represents a foundational pillar of the life sciences. However, the very complexity of the samples cellular structure leads to one of the main limitations of optical microscopy: light scattering confines it to the outer most tissue layers. In the past decades, multiphoton microscopy successfully extended the accessible depth ranges and on a different front, advances in optical wavefront shaping showed that scattering can be compensated for, even in regimes where light fully lost its initial directionality. The aim of this action is to establish scattering correction through wavefront shaping in higher-order multiphoton microscopy, namely three-photon fluorescence imaging, to surpass the scattering barrier and reach unprecedented depths in optical microscopy.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
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Programme(s)
Funding Scheme
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinator
75006 Paris
France