We have developed Lamp tests for four economically significant diseases affecting bananas. Instead of focusing on a single pathogen, our approach considers the characteristics of the pathogens from the outset of research, leading to the development of a common extraction protocol applicable before each specific Lamp test. Our efforts extend beyond the development of LAMP tests, as we have implemented the entire process from sample collection to the final diagnostic result, with validation in the field and through interlaboratory tests. Protocols have been transferred to Qualiplante company for ready-to-use kits manufacturing and MOKO and FocTR4 kits are already commercially available. We have also developed a multiplex LAMP assay for MOKO and FocTR4, as these diseases can be found in the same countries and plots. This molecular tool will be validated in the future.
Furthermore, we are pioneering in the development of an innovative diagnostic tool, RPA-Crispr Cas12a, for FocTR4 strains detection. This technique is relatively uncommon in plant pathology, marking a significant "proof of concept" that demonstrates the feasibility of developing and utilizing such tools in plant health.
This work on LAMP development will help raise awareness of this technique and will demonstrate its usefulness for a quick and reliable diagnosis. Future steps will consist of integrating these protocols into European and international diagnostic standard methods.
The research outputs will primarily contribute to improving pathogen control by providing efficient and valuable tools to improve pathogen surveillance within territories and at borders as well as promoting the use of disease-free material for planting. Moreover, through training and information dissemination, this research will strengthen the capacity and skills of national plant protection services and organizations involved in surveillance.
Accurate pathogen detection can result in significant economic benefits, usually achieved through reduced pesticide misuse and increased crop production and quality due to properly timed interventions. International trade will also benefit, as these diagnostic tools will support safe, pathogen-free trade and ensure compliance with the sanitary and phytosanitary requirements of importing countries. Therefore, our research contributes to the global transition toward sustainable food systems promoted by Europe, enhancing crop protection, agricultural productivity, food security, and even human and environmental health by potentially reducing pesticide use.
