StructUral ProtEins foR Biomedical materials

Descripción del proyecto

Polímeros como fuente de materiales biomédicos avanzados

Los polímeros son una fuente de materiales biomédicos avanzados gracias a su abundancia natural, su degradación ajustable, su fácil procesamiento, su citocompatibilidad y sus propiedades físico-químicas controlables. La cuestión ahora es cómo las características de los polímeros y las condiciones de procesamiento determinan la nanoestructura de los materiales derivados. El proyecto financiado con fondos europeos SUPERB, utilizará simulaciones de dinámica molecular para seleccionar secuencias de proteínas y condiciones de procesamiento para la fabricación de materiales. También biosintetizará las proteínas seleccionadas a través de un proceso fermentativo. Además, el proyecto fabricará, probará y caracterizará hidrogeles o materiales de película delgada para la validación de los modelos. SUPERB desarrollará asimismo una metodología para mejorar la racionalidad y acortar el tiempo necesario para fabricar nuevos materiales biomédicos derivados de proteínas, lo que reducirá el uso de animales y el coste experimental.

Objetivo

The SUPERB project will train an experienced researcher (ER), Dr. Diego López Barreiro, in the modelling and manufacture of protein-based polymers for healthcare materials. The ER will combine building blocks of structural proteins like elastin or collagen into de novo fusion protein polymers. The healthcare community is becoming increasingly interested in these polymers as a source of advanced healthcare materials, due to their natural abundance, tuneable degradation, easy processability, cytocompatibility, or controllable physicochemical properties. This project will study how polymer features and processing conditions determine the nanostructure of the materials derived thereof, and hence their macroscopic properties. This will be done through an interdisciplinary approach that will (i) use molecular dynamics simulations to select protein sequences and processing conditions for material manufacture; (ii) biosynthesise selected proteins via fermentative processes; and (iii) fabricate, test and characterise hydrogels or thin film materials for model validation. The excellent knowledge and expertise of the host institution in this crucial field for the EU will ensure the success of this proposal. This project will develop a methodology to improve the rationality and decrease the time needed to develop new protein healthcare materials, reducing animal use and experimental costs. Moreover, while the proposed approach is used here on a limited suite of protein building blocks, it can be applied to any other peptide sequences or biopolymers (e.g. bioactive peptides, bioplastics, nanocellulose), becoming a valuable tool to accelerate the discovery of healthcare materials. Furthermore, the project will support the ER in deepening his expertise in protein materials and expose him to the industrial field. This will also enhance his research, project and IPR management skills, acquiring necessary competences for professional maturity and an independent career.