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Coupling of Optical tweezers with Tip-enhanced Raman Spectroscopy for single-molecule investigation of supramolecular systems

Project description

Twofold system opens a window into supramolecular bonding

Supramolecular structures are very large molecules formed by grouping or bonding smaller molecules together. In contrast to conventional chemistry and covalently bonded atoms, supramolecular chemistry typically involves weaker and reversible interactions like those that are fundamental to biological systems and functions. Technological advances have made it possible to evaluate the mechanical strength of non-covalent interactions in the single-molecule regime. However, chemical characterisation has so far been impossible. The EU-funded TweeTERS project is combining optical tweezers for mechanical insight with cutting-edge tip-enhanced Raman spectroscopy for chemical insight. The setup will enable the simultaneous measurement of reversible binding events and the identification of the molecular species involved, the inter- and intramolecular interactions and their orientations for single-molecule manipulation of supramolecular systems with unprecedented spatial resolution.

Objective

Quantitative information on the dynamics and mechanistic principles behind supramolecular systems can be investigated in the single-molecule regime by measuring the nanometer displacements resulting from the application of picoNewton forces in optical trapping (OT) experiments. In particular, the mechanical strength of non-covalent interactions can be quantified when studying the reversible breaking/formation of hydrogen bonds on individual host-guest systems or the switching of a macrocycle between binding stations in a molecular shuttle. However, the fundamental chemical mechanisms behind the obtained real-time operational kinetics are not accessible with OT experiments and fundamental questions about the physicochemical processes underlying real-operation remain unanswered.
The goal of this project is to merge OT for single-molecule optical force microscopy experiments with tip-enhaced Raman spectroscopy (TERS). TERS is a powerfull nearfield-based techinque based on the coupling of Raman spectroscopy and scanning probe microscopy. It provides chemical characterization with single molecule sensitivity and few-nm spatial resolution. Since Raman spectroscopy is sensitive to molecular species, inter and intra molecular interactions and orientations; by combining OT-optical force microscopy with TERS, we will access the underlying physicochemical processes triggering specific shuttling events in molecular motors and other types of supramolecular systems. In particular, we aim to create a hybrid tool that can disentangle the relation between mechanical, conformational and chemical properties of individual synthetic supramolecular systems and the non-covalent interactions governing their behavior, with single-molecule sensitivity and spatial resolution in the range of 10 nm. TweeTERS will lead to a major technological improvement in the single-molecule manipulation field and in particular, in the nearly un-explored field of single-molecule supra-molecular chemistry.

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2019

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Coordinator

FUNDACION IMDEA NANOCIENCIA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 160 932,48
Address
CALLE FARADAY 9 CIUDAD UNIVERSITARIA DE CANTOBLANCO
28049 Madrid
Spain

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Comunidad de Madrid Comunidad de Madrid Madrid
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 160 932,48
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