Project description
Shedding light on photoactivation events in energy-flexible bacteria
Despite all we know about photosynthesis, there are many details of the complex signal transduction pathways that remain to be illuminated. This becomes even more complex in 'facultative' photosynthetic organisms in which photosynthesis is optional – in other words, they can switch energy sources for survival. The expression of photosynthetic genes in the facultatively photosynthetic bacterium Rhodobacter sphaeroides is controlled by both oxygen and light levels. For more than two decades, scientists have been studying two photoreceptor proteins, AppA and CryB, involved in this regulation. However, it is still unclear how changes in these proteins in response to light affect the downstream signalling cascade. The EU-funded DynAppACry project plans to shed light on these light-induced events with the application of high-tech biochemical, computational, spectroscopic and structural methods. The resulting time-resolved molecular 'movie' could help us figure out how to use light to non-invasively control biological processes.
Objective
Photosynthetic organisms need to sense environmental light effectively in order to regulate cellular processes. AppA (Activation of Photopigment and PUC A protein) and CryB (cryptochrome B) are two flavin (FAD) containing photoreceptors found in the facultatively photosynthetic bacterium Rhodobacter sphaeroides and depending on the oxygen levels can regulate the expression of photosynthetic genes by light. Photoexcitation of AppA and CryB is accompanied by changes on their conformation and their affinity to partner binding proteins to initiate signaling transduction processes. The mechanisms by which AppA and CryB regulate downstream signaling events is not known. Thus there is substantial interest in capturing the conformational landscape that lead to the signaling state and in understanding how absorption of light by the flavin is coupled to these conformational changes. The aim of the project is to provide atomic level characterization of the photoactivation mechanism of AppA and structural information on the photocycle of CryB. To achieve our objectives, we will use a combination of biochemical methods, ultrafast transient absorption spectroscopy, QM/MM (hybrid quantum mechanics/molecular mechanisms) calculations and time-resolved structural methods. The latter include TR-serial crystallography (SX) at synchrotrons (SSX) and X-ray free electron lasers (SFX) and TR-solution scattering (small-angle, SAXS and wide-angle X-ray scattering, WAXS). Ultimately, this project will provide a molecular movie of the AppA that features the structural changes occurring upon blue-light illumination and significant information on the photocycle of CryB. The proposed research lies on the implementation of new instrumentation and novel approaches to advance our knowledge on how photoreceptors function in order to engineer novel systems that use light as a tool to achieve noninvasive control of biological processes with high spatiotemporal resolution.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences physical sciences quantum physics
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy absorption spectroscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75015 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.