Description du projet
Une thérapie basée sur les nanoparticules pour le cancer du sein
Le cancer du sein triple négatif (CSTN) représente près de 20 % de tous les cas de cancer du sein et se caractérise par son agressivité et son faible taux de survie. Comme la plupart des thérapies échouent, le projet HypoCyclo, financé par l’UE, recherche des alternatives innovantes. Les chercheurs proposent de trouver une combinaison optimale de chimiothérapie et de thérapie photodynamique (TPD) basée sur les nanoparticules. Parallèlement à des médicaments efficaces comme le paclitaxel, les nanoparticules transporteront des photosensibilisateurs et des agents libérant de l’oxygène pour lutter contre l’hypoxie tumorale et améliorer l’efficacité du traitement. L’idée consiste à combiner l’administration de nanoparticules avec des lasers et des fibres optiques qui facilitent l’apport de lumière interstitielle et peropératoire. Les chercheurs envisagent un protocole de synthèse de nanoparticules simple pour assurer une production à faible coût et donc à grande échelle.
Objectif
Among all breast cancers (BC) affecting female population worldwide, 20% is represented by Triple Negative Breast Cancer (TNBC), an aggressive subtype with very limited therapeutic options and poor survival. Seeking for innovative TNBC therapies, HypoCyclo aims at the optimal combination of chemotherapy and photodynamic therapy (PDT). We envisage novel, tailored cyclodextrin (CyD)-based polymeric nanoparticles (NPs) able to load “known” therapeutic agents and decorated with a photosensitizer as well as an Oxygen-Releasing Agent. HypoCyclo focuses on the very effective drug paclitaxel (PCX) presenting several treatment problems like water insolubility. Biocompatible, novel CyD polymers are expected to solubilize and encapsulate through non-covalent binding the drug of choice in order to implement PCX chemotherapy with PDT, through the decoration with a PS able to generate antitumor reactive oxygen species (ROS) in situ. Indeed, PDT has become a very challenging yet feasible option thanks to the technological advances in lasers and fiber-optics allowing interstitial and intra-operative light delivery for the treatment of solid tumours, including BC. Finally, as hypoxia in BC tissue compromises standard chemotherapy and PDT, we will tailor our NPs with an ORA able to release oxygen in cells, enabling an alternative ROS source. The PCX loaded NPs will be completely characterized and tested in 2D and 3D TNBC models. Our goal is to keep the synthetic protocols simple, low-cost and green to facilitate industrial upgrade of the loaded multimodal NPs endowed with improved in vitro efficacy. Two partners, the SME Cyclolab and UNIBO, both already collaborating with the supervisor, will give the necessary support in mixed-CyD polymer synthesis and in the biological assays, respectively, guaranteeing a successful outcome. The project foresees a demanding training program for ER that will help him to become an independent researcher with a complete profile.
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinateur
00185 Roma
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