Project description DEENESFRITPL Decoding bristle formation in worms Segmented worms have bristle-like projections on their surface known as chaetae. These extracellular structures can display a diverse array of complex shapes and are formed following an interplay between the cytoskeleton and controlled polymerisation of chitin, a glucose-based polymer. The EU-funded CHAETA project aims to advance knowledge of this process by investigating the molecular mechanisms underlying chaetae synthesis, focussing on chitin synthases. Scientists will use the bone-eating worm Osedax japonicus as a model organism, and by combining gene-editing, microscopy and cutting-edge transcriptomic techniques they will unveil the genetic network regulating chaetogenesis. Since the process is comparable to 3D printing, the results of the study extend beyond biology and could be exploited by scientists studying biomechanics and biomineralisation. Show the project objective Hide the project objective Objective Chaetae, the chitinous bristles, of segmented worms are without a doubt one of their most characteristic features. A single cell, called chaetoblast, forms these complex extracellular structures, through an intricate interplay of the cytoskeleton and controlled polymerization of chitin. I have been investigating this fascinating system and published a series of papers describing the process of chaetal formation in various annelids using serial TEM and histology. With this proposed project I will combine my previous training and take the next logical step in the study of chaetogenesis; by investigating the molecular underpinnings of chaetal formation, focusing on chitin synthases. Chitin is one of the most dominant biopolymers in nature and a key building block of diverse extracellular structures. The bone eating worm Osedax japonicus will be the main focus of this study. Its fast and simple chaetal development, together with the drastic anatomical difference between males and females, renders it a perfect model to link the differential expression of chitin synthases to any relevant point of chaetogenesis that can be ultrastructurally profiled. Genetically well-examined annelid Capitella teleta will serve to test the functional genetics of chitin synthases using CRISPR/CAS9. This integrative approach combining advanced microscopical techniques with cutting-edge transcriptomics will result in a giant leap towards revealing the genetic network regulating chaetogenesis. I will be laying the groundwork to fully understand how morphologically complex, chitinous hard structures can be formed by a single cell. Elucidating the molecular and cellular mechanisms behind this process, that is comparable to a biological 3D printer, is highly relevant for not only annelid researchers but also to a broader scientific community studying biomechanics, biomineralization, cell-biology and even bionics. Fields of science natural sciencesbiological sciencesgeneticsnatural sciencesbiological sciencesbiophysicsnatural sciencesbiological scienceshistologynatural sciencesbiological scienceszoologyinvertebrate zoology Keywords Organogenesis Chaetogenesis Morphogenesis Annelida Ultrastructure Chitin Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2019 - Individual Fellowships Call for proposal H2020-MSCA-IF-2019 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator KOBENHAVNS UNIVERSITET Net EU contribution € 207 312,00 Address Norregade 10 1165 Kobenhavn Denmark See on map Region Danmark Hovedstaden Byen København Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
