In order to develop a rice base material resistant to the clethodim herbicide, firstly, mutation application was carried out. Then low dose resistance study, high-dose resistance study, and determination of mutation points study were carried out. In the mutation generation study, the standard protocol for EMS mutagenesis application in rice was developed. It is useful to obtain a sufficient number of mutant plants instead of working with a severe mutation in a few plants. This application protocol contains presoaking for 12 hours, 0.5% dose EMS application for six hours, final washing for six hours, and dry for 72 hours. According to the created protocol, the chemical mutation was applied to 5 rice varieties and a low-dose clethodim resistance study was started. Chemical mutation applied seeds 10,000 seeds each of 5 rice varieties, were planted in the plant growth chamber. It was known that clethodim herbicide can be killed the rice, however, the doses needed to be standardized. Therefore, a dose-response study was carried out with clethodim in rice. As a result of the study, the dose that killed 90% (ED90) of the rice was determined in the range of 70-80 g ai ha-1. Clethodim 1x was utilized 150 gr ai ha-1 in the experiments. In the low-dose experiment, herbicide was applied at 1/2x doses on the m1 plants. Clethodim was applied when the plants were at the 3-4 leaf stage in the spray chamber. Visual injury ratings were conducted 28 days after treatment (DAT) based on 0=not injury and 100=plant killed. A total of 30 plants survived. The frequency of survival was found as 0.06%. Then, the mutation determination process started. Clethodim herbicide binding point was on the ACCase carboxtransferase region on the 5th chromosome in rice. Five primers were designed to cut this region. With this primer, the target region was cut and amplified by PCR and sequenced by the Sanger method. Although the plants were found to be resistant, it was observed that the original sequence was not changed in the target region. In the high dose resistance study, the mutation was applied to 10000 seeds of 5 varieties according to the determined protocol. First-year mutant rice was planted and generation advancement was achieved from M1 to M2 plant. The M2 generation was grown in the field and harvested for herbicide scanning. The second-year M2 mutant rice plant was grown up 3-4 leaf stage on the field. Double doses of clethodim were sprayed on mutant rice varieties two times when first at the 3-4 leaf stage and second 14 days later the first application, and 750 surviving plants were selected. The clethodim resistance was tested in the greenhouse at fully controlled conditions, such as an automatic herbicide sprayer and arranged climatic conditions, and 3 out of 750 M3 plant genotypes survived high-dose clethodim treatment. At the next stage observed that these 3 genotypes had no resistance exceeding ½ × dose detected in the herbicide dose-response study and they had no mutation in the ACCase gene region according to the DNA sequence study. As a result, 30 low-dose resistant genotypes were developed, but no material was determined at high-dose resistance for commercial resistance. The dynamic structure of the study allows us to test the newly produced material again until the study is terminated. High-dose resistance study will be re-implemented to achieve success.