Project description
Exosomes as nanomessengers in assisted reproductive technologies
There is increasing evidence of the role of oviduct exosomes (EXs) as mediators of early maternal signalling. The absence of communication with the maternal signalling in the in vitro embryo production (IVP) system might explain the low quality of these embryos in comparison to the ones developed in the physiological conditions (in vivo). Recent data revealed the oviduct-derived EXs' protein signature and their role in embryo–oviduct interactions. The EU-funded EXORep project will employ the bovine model to evaluate whether the presence, quality and the stage of the early embryo modulate EX proteins as well as mRNA and miRNA content. It will also assess the effect of in vitro culture with EXs on embryo development and quality. The obtained results may be relevant to improve (IVP) system, including the human assisted reproductive technologies.
Objective
The current embryo in vitro culture conditions used in most laboratories of assisted reproductive technologies (ARTs) allow the embryo to develop to the blastocyst stage and to achieve pregnancy. However, the quality of those embryos is suboptimal compared to the in vivo ones and the obtained pregnancy rates are low. The lack of maternal signals, during the in vitro period has been postulated as a main reason for these suboptimal results. There is increasing evidence of the role of the oviduct exosomes (oEXs) as mediators of the early maternal-cross talk. Recent data revealed the first oviduct-derived EXs protein signature and their important roles in embryo-oviduct interactions. In the present proposal, using bovine model, we aimed to: (i) Evaluate whether the presence of the early embryo in the oviduct could modulate the oEXs proteins, mRNA and miRNA content depending on its stage of development. (ii) Assess whether the embryo quality (good or poor embryo) affect the content of EXs secreted in vitro by the oviduct epithelial cells. (ii) Assess the effect of in vitro culture with oEXs on the embryo development and quality. To accomplish these tasks, an ambitious, realistic, work plan has been designed based on the excellent background of the Fellow, and the training she will receive, the expertise of the supervisor in female reproductive physiology and the infrastructure within the host lab for conducing all the experiments with most appropriate tools. This will ensure that the Fellow completes the scientific objectives of the proposal, communicate and disseminate the results to the scientific and public, while acquiring new set of skills that will have an important impact in her career development. If the beneficial effect of oEXs on embryo development is recapitulated in cattle, it could improve in vitro embryo production largely used in ART in dairy cattle industries. Moreover, the obtained results may be transferred in human species
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology
- medical and health sciences clinical medicine obstetrics
- medical and health sciences basic medicine physiology
- agricultural sciences animal and dairy science domestic animals animal husbandry
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
8006 Zurich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.