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Leaf-inspired nanocellulose frameworks for next generation photosynthetic cell factories

Resultado final

Data management plan

Document which describes how data produced in the course of the project is deposited, handled and managed during and after the end of the project.

Optimized transport properties in the matrix to support prolonged cell viability

Matrix materials which display such transport properties of gas, nutrients, light and water that support cell life-time for long periods of time available.

Simple passive cells entrapment methods

Strategies to entrap cells with passive immobilisation approaches are available.

A palette of nanocellulose grades and crosslinkers ready

Various grades of nanocelluloses and crosslinkers available in order to improve mechanical performance of matrix and facilitate the interactions between cells and the matrix,

Photoautotrophic production strains characterized in a reference solid system

Reference solid production system based on alginate and photoautotrophic strains available

Website, logo, flyers, social media profiles

Deliverable reports that website and project logo are available. Other communication channels related to social media are established (especially LinkedIn and Twitter)

Risk Management Plan (RMP)

Risk management plan for the project.

Dissemination, exploitation and communication plan

A plan how to disseminate, exploit and communicate the project outcome available.

Risk Management Plan (RMP) update

Risk management plan for the project.

Conditions and protocols for the analytics of product formation

Deliverable collects the conditions and characterisation protocols of the production strains with respect to their productivity.

Kick-off meeting report

Short report which announces that project has started.

Water induced effects on matrix physical performance characterized

Report delivers how presence of water alters the structural features, especially porosity and mechanical performace of the matrix materials.

Dissemination, exploitation and communication plan update

An updated plan how to disseminate, exploit and communicate the project outcome available.

Dissemination, exploitation and communication plan 2nd update

An updated plan how to disseminate, exploit and communicate the project outcome available via all the defined channels (publications, presentations, workshops, news and newsletters distributed via social media and conventional media, exhibitions, public events etc.)

Publicaciones

Regulatory electron transport pathways of photosynthesis in cyanobacteria and microalgae: Recent advances and biotechnological prospects

Autores: Lauri Nikkanen, Daniel Solymosi, Martina Jokel, Yagut Allahverdiyeva
Publicado en: Physiologia Plantarum, 2021, ISSN 0031-9317
Editor: Blackwell Publishing Inc.
DOI: 10.1111/ppl.13404

Photobiocatalytic Oxyfunctionalization with High Reaction Rate using a Baeyer–Villiger Monooxygenase from <i>Burkholderia xenovorans</i> in Metabolically Engineered Cyanobacteria

Autores: Erdem, Elif; Malihan-Yap, Lenny; Assil-Companioni, Leen; Grimm, Hanna; Barone, Giovanni Davide; Serveau-Avesque, Carole; Amouric, Agnes; Duquesne, Katia; de Berardinis, Véronique; Allahverdiyeva, Yagut; Alphand, Véronique; Kourist, Robert
Publicado en: ACS Catalysis, Edición 12, 2022, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.1c04555

Design and Investigation of a Photobiocatalytic setup for efficient biotransformations within recombinant cyanobacteria in continuous flow

Autores: Alessia Valotta, Lenny Malihan-Yap, Kerstin Hinteregger, Robert Kourist, Heidrun Gruber-Woelfler
Publicado en: ChemSusChem, 2022, ISSN 1864-564X
Editor: Wiley-VCH GmbH
DOI: 10.1002/cssc.202201468

Mapping Nanocellulose- and Alginate-Based Photosynthetic Cell Factory Scaffolds: Interlinking Porosity, Wet Strength, and Gas Exchange

Autores: Tuukka Levä, Ville Rissanen, Lauri Nikkanen, Vilja Siitonen, Maria Heilala, Josphat Phiri, Thaddeus C. Maloney, Sergey Kosourov, Yagut Allahverdiyeva, Mikko Mäkelä, Tekla Tammelin
Publicado en: Biomacromolecules, 2023, ISSN 1525-7797
Editor: American Chemical Society
DOI: 10.1021/acs.biomac.3c00261

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli.

Autores: Gábor Szilveszter Tóth, Vilja Siitonen, Lauri Nikkanen, Lucija Sovic, Pauli Kallio, Robert Kourist, Sergey Kosourov & Yagut Allahverdiyeva
Publicado en: Biotechnology for Biofuels and Bioproducts, 2022, ISSN 2731-3654
Editor: BioMed Central Ltd
DOI: 10.1186/s13068-022-02248-1

Nanocellulose-based mechanically stable immobilization matrix for enhanced ethylene production: a framework for photosynthetic solid-state cell factories

Autores: V. Rissanen, S. Vajravel, S. Kosourov, S. Arola, E. Kontturi, Y. Allahverdiyeva, T. Tammelin
Publicado en: Green Chemistry, Edición 23/10, 2021, Página(s) 3715-3724, ISSN 1463-9262
Editor: Royal Society of Chemistry
DOI: 10.1039/d1gc00502b

Photosynthetic hydrogen production: Novel protocols, promising engineering approaches and application of semi‐synthetic hydrogenases

Autores: Sergey Kosourov, Maximilian Böhm, Moritz Senger, Gustav Berggren, Karin Stensjö, Fikret Mamedov, Peter Lindblad, Yagut Allahverdiyeva
Publicado en: Physiologia Plantarum, 2021, ISSN 0031-9317
Editor: Blackwell Publishing Inc.
DOI: 10.1111/ppl.13428

Engineered green alga Chlamydomonas reinhardtii as a whole-cell photosynthetic biocatalyst for stepwise photoproduction of H2 and ε-caprolactone

Autores: Vilja Siitonen, Anna Probst, Gábor Szilveszter Tóth, Robert Kourist, Michael Schroda, Sergey Kosourov, Yagut Allahverdiyeva
Publicado en: Green Chemistry, 2023, ISSN 1463-9270
Editor: Royal Society of Chemistry
DOI: 10.1039/d3gc01400b

Towards the rate limit of heterologous biotechnological reactions in recombinant cyanobacteria

Autores: Giovanni Davide, Barone; Michal, Hubáček; Lenny, Malihan-Yap; Hanna C, Grimm; Lauri, Nikkanen; Catarina C, Pacheco; Paula, Tamagnini; Yagut, Allahverdiyeva; Robert, Kourist
Publicado en: Biotechnology for Biofuels and Bioproducts, 2023, ISSN 2731-3654
Editor: Springer Nature
DOI: 10.1186/s13068-022-02237-4

Bioinspired mechanically stable all-polysaccharide based scaffold for photosynthetic production

Autores: Tuuli Virkkala, Sergey Kosourov, Ville Rissanen, Vilja Siitonen, Suvi Arola, Yagut Allahverdiyeva, Tekla Tammelin
Publicado en: Journal of Materials Chemistry B, 2023, ISSN 2050-7518
Editor: Royal Society of Chemistry
DOI: 10.1039/d3tb00919j

Tuning the porosity, water interaction and redispersion of nanocellulose hydrogels by osmotic dehydration

Autores: Valentina Guccini; Josphat Phiri; Jon Trifol; Ville Rissanen; Seyede Maryam Mousavi; Jaana Vapaavuori; Tekla Tammelin; Thaddeus Maloney; Eero Kontturi
Publicado en: ACS Applied Polymer Materials, 2022, ISSN 2637-6105
Editor: American Chemical Society
DOI: 10.1021/acsapm.1c01430

Balancing photosynthesis, O<sub>2</sub> consumption, and H<sub>2</sub> recycling for sustained H<sub>2</sub> photoproduction in pulse-illuminated algal cultures

Autores: Sindhujaa Vajravel; Yagut Allahverdiyeva; Sergey Kosourov
Publicado en: Sustainable Energy & Fuels, Edición 12, 2023, ISSN 2398-4902
Editor: Royal Society of Chemistry
DOI: 10.1039/d2se01545e

Internal Illumination to Overcome the Cell Density Limitation in the Scale‐up of Whole‐Cell Photobiocatalysis

Autores: Markus Hobisch, Jelena Spasic, Lenny Malihan‐Yap, Giovanni Davide Barone, Kathrin Castiglione, Paula Tamagnini, Selin Kara, Robert Kourist
Publicado en: ChemSusChem, Edición 14/15, 2021, Página(s) 3219-3225, ISSN 1864-5631
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.202100832

Recent Advances in Cyanobacterial Biotransformations

Autores: Lenny Malihan-Yap, Hanna C. Grimm, Robert Kourist
Publicado en: Chemie Ingenieur Technik, 2022, ISSN 1522-2640
Editor: Wiley-VCH GmbH
DOI: 10.1002/cite.202200077

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