Periodic Reporting for period 1 - FAST_TOX (BANKED HEPATOCYTE MONOLAYERS FOR ACCELERATING DRUG TOXICITY SCREENING)
Período documentado: 2020-09-01 hasta 2023-02-28
Our core technology is based upon macromolecular cryoprotectants – polymers which can mitigate cold stress, using mechanism of action that standard, organic solvent, based cryoprotectants cannot. Firstly we demonstrated that cell monolayers of 3 different cell types can be cryopreserved as 2D monolayers leading to post-thaw recovery of the cells, with up to 2 fold increase compared to current technologies. This has also been extended to primary hepatocytes, which are in large demand for toxicity testing but do not always recover well post-thaw. Finally a challenging 3D spheroid model was cryopreserved, showing high recovery. For all cells (2 and 3D) model toxicological challenges confirmed they are suitable for drug screening. The results of this are now being commercialised in partnership with Cryologyx Ltd.