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Understanding Cerebral Malaria using 3D Blood-Brain Barrier models

Project description

3D model of blood-brain barrier to study cerebral malaria pathology

Malaria is a major public health concern with more than 400 000 deaths per year. Cerebral malaria (CM) is characterised by sequestration of infected red blood cells in the brain microvasculature, blood-brain barrier (BBB) disruption and brain swelling, resulting in high mortality. The EU-funded Mal3D-BBB project aims to model the human CM pathology with cutting-edge in vitro bioengineering approaches. The researchers will develop BBB models with 3D microfluidic networks that incorporate multiple cell types: brain microvascular endothelial cells, astrocytes and pericytes. The developed platform aims to recreate physiological BBB permeability rates, and will be used to understand the molecular mechanisms of BBB disruption after P. falciparum sequestration, and whether parasite and host factors synergise to increase pathology.

Objective

Malaria is a major public health problem and it still causes more than 400,000 deaths per year. Cerebral malaria (CM) is one of the most serious complications, with 20% mortality rates even after administration of fast-acting antimalarials. CM pathology is characterized by sequestration of P. falciparum-infected red blood cells (iRBC) in the brain microvasculature, blood-brain barrier (BBB) disruption, and brain swelling.
Our current knowledge of CM is primarly based on autopsy studies, because of the absence of suitable animal models. However, there are numerous pathogenic aspects that cannot be studied from post-mortem samples, such as disease progression. In Mal3D-BBB, we bypass these limitations by recreating the human CM pathology with cutting-edge in vitro bioengineering approaches. Rather than using 2D endothelial monolayers, we will develop BBB models with 3D tubular geometry that incorporate multiple cell types: brain microvascular endothelial cells, astrocytes and pericytes. We will mimic vessel dimensions and flow dynamics of the brain vasculature with the goal to recreate physiological BBB permeability rates. Using such technology brings a unique angle to malaria research to evaluate in a controlled and systematic way 1) the molecular mechanisms of BBB disruption after P. falciparum sequestration, and 2) whether parasite and host factors synergize to increase pathology. The findings obtained by this cutting-edge technology will be further validated in samples from CM patients, whose neurovascular pathology has been thoroughly characterized using MRI.
Our interdisciplinary approach aims to provide a holistic understanding of CM malaria pathogenesis. In return, this knowledge will identify new pathways that could be counteracted to develop therapies to reduce patient mortality. In a broader context, we will build an innovative platform that captures the complex physiology of the BBB, and can be translated to the study of other neurovascular diseases.

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Topic(s)

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ERC-STG - Starting Grant

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Call for proposal

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(opens in new window) ERC-2020-STG

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Host institution

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 492 900,00
Address
Meyerhofstrasse 1
69117 Heidelberg
Germany

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Region
Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 492 900,00

Beneficiaries (1)

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