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In situ DNA sequencing-based microscopy for subcellular spatial transcriptomics

Project description

A revolutionary sequencing-based microscopy technology

During the early stages of zygote formation, the reservoir of maternal RNA is replaced by the transcription of zygotic genes. To understand the complexity of transcriptional regulation in the embryo, it is necessary to develop new tools for spatial transcriptomics. The EU-funded DNA_MICROSCOPY project will build on a pre-existing 2D topological DNA microscopy approach that performs in situ PCR and next-generation sequencing. Researchers aim to perform in situ whole-transcriptome sequencing in 3D with subcellular resolution and combine it with protein analysis. The novel technology will help dissect key events of early development, such as the determination of cell polarity and fate during cleavage.

Host institution

KUNGLIGA TEKNISKA HOEGSKOLAN
Net EU contribution
€ 1 499 947,00
Address
Brinellvagen 8
100 44 Stockholm
Sweden

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Region
Östra Sverige Stockholm Stockholms län
Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00

Beneficiaries (1)

KUNGLIGA TEKNISKA HOEGSKOLAN
Sweden
Net EU contribution
€ 1 499 947,00
Address
Brinellvagen 8
100 44 Stockholm

See on map

Region
Östra Sverige Stockholm Stockholms län
Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00