A revolutionary sequencing-based microscopy technology
During the early stages of zygote formation, the reservoir of maternal RNA is replaced by the transcription of zygotic genes. To understand the complexity of transcriptional regulation in the embryo, it is necessary to develop new tools for spatial transcriptomics. The EU-funded DNA_MICROSCOPY project will build on a pre-existing 2D topological DNA microscopy approach that performs in situ PCR and next-generation sequencing. Researchers aim to perform in situ whole-transcriptome sequencing in 3D with subcellular resolution and combine it with protein analysis. The novel technology will help dissect key events of early development, such as the determination of cell polarity and fate during cleavage.
Fields of science
Funding SchemeERC-STG - Starting Grant
100 44 Stockholm
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