In situ DNA sequencing-based microscopy for subcellular spatial transcriptomics

Project description

A revolutionary sequencing-based microscopy technology

During the early stages of zygote formation, the reservoir of maternal RNA is replaced by the transcription of zygotic genes. To understand the complexity of transcriptional regulation in the embryo, it is necessary to develop new tools for spatial transcriptomics. The EU-funded DNA_MICROSCOPY project will build on a pre-existing 2D topological DNA microscopy approach that performs in situ PCR and next-generation sequencing. Researchers aim to perform in situ whole-transcriptome sequencing in 3D with subcellular resolution and combine it with protein analysis. The novel technology will help dissect key events of early development, such as the determination of cell polarity and fate during cleavage.

Fields of science

Host institution

KUNGLIGA TEKNISKA HOEGSKOLAN

Net EU contribution

€ 1 499 947,00

Address

Brinellvagen 8
100 44 Stockholm
Sweden

Region

Östra Sverige Stockholm Stockholms län

Activity type

Higher or Secondary Education Establishments

Links

Contact the organisation Website

Participation in EU R&I programmes

HORIZON collaboration network

Other funding

€ 0,00

Beneficiaries (1)