Periodic Reporting for period 1 - ENSEMBLE (Structure and functions of the brain extracellular space)
Período documentado: 2021-05-01 hasta 2022-10-31
Despite these hurdles, we are comparably less affected, although we did not hire as many high profiles as we would have liked. The purchase of most types of equipment has eventually been completed (CNRS, INSERM), enabling it to set up the so-called Woodstock single particle tracking system entirely. To complete the second SUSHI system, some final pieces must be purchased (University of Bordeaux).
The two systems, proprietary of the ENSEMBLE project, are established within the dedicated 150 m2 open lab (refurbishment completed as per plan, most hired staff working into that project-dedicated environment) and are due to merge at some point to enable the dual mode of operation for the in vivo imaging of both SUSHI and SWCNT tracking. We have launched web-based working and information-sharing tools and established weekly topic-oriented meetings (small groups discussing operational details) with an additional monthly general meeting (project-wide meetings to share science and orientations).
The expected results until the end of the project are the following:
i) Defining the ECS characteristics in brain slices (hippocampus, cortex, striatum...) from organotypic and acute preparations.
ii) Defining the ECS in vivo in the cortex of mice using both SPT and SUSHI-based methods
iii) Defining the movement of extracellular proteins, such as autoantibodies, in the ECS of brain areas
iv) Defining the ECS in models of neurodegenerative diseases.