The activities in AquaSPICE have been continued in the second reporting period with technical implementations in the Case Studies (CSs) and developments in digital tools. The conceptual Water Efficiency Framework has been developed and tested with several CSs. For the technical activities, the IMPROVED containers continued to allow the investigation different treatment technologies including ion exchange and membrane filtration such as ultrafiltration and reverse osmosis. The containers, used on the water-stressed Dow’s sites in Boehlen and Terneuzen, treated different water streams and demonstrated the feasibility of treatment and reuse options. In the Italian CS, focusing on a hydrogen peroxide production plant, the pilot scale system using membrane bioreactor (MBR) technology has been implemented and initial tests have been performed. In the Belgian CS, data collection continued based on the Real-Time Monitoring (RTM) network of 45 sensors installed along the Albert canal upstream of the Antwerp harbour. The second pillar of the CS involves the industrial cooling processes, investigated in the MERADES pilot, and resource recovery options at BASF, where the IMPROVED containers have been deployed and extensive tests have been performed. In the Romanian CS, pilot tests have been implemented on-site with an advanced wastewater treatment process including MBR technology and UV disinfection with water quality monitoring by an innovative online flow-cytometry. In the Turkish CS, for the very challenging wastewater streams from the oil refinery in Izmit, a pilot system with granular sludge has been implemented. The core digital offering of AquaSPICE, the WaterCPS, aims to provide advanced water-saving awareness and optimised water efficiency at different industrial levels by synthesising digital twins of industrial and value chain entities. All digital components have been designed, developed, and deployed in most CSs. The RTM platform has been developed and sensors have been deployed in all CSs. The deployment of the RTM platform was completed for all CSs, except for Italian and Turkish CSs, that is in progress. The dynamic modelling of the factory and value chain processes as well as the simulation techniques support this challenge, together with the advanced data-driven analytic tools, AI methods, optimisation techniques and dynamic Life Cycle Assessment (LCA). The digital twins have been initialised for all CSs. All intelligent services (simulation, analytics, optimisation and LCA) are being integrated into the WaterCPS platform. The dissemination component has already provided a wealth of open information through various channels including a website, social media, and a digital newsletter. AquaSPICE has been presented in various workshops and digital events and several publications have been prepared. AquaSPICE pursues a very intensive interaction with a range of stakeholders on the European and site-specific level to pave the way for an impact-oriented project realisation.