Objetivo
The solid state fermentation process (SSF) is well known in traditional food preparations and for such applications as fodder silage and composting.
The basic purpose of the proposed project is to apply SSF, using sugar beet pulp as the main substrate, for the manufacture of medium or high added value products. Previous experiences have shown the feasibility of such an operation for the production of protein enriched pulp and industrial enzymes. The production of pectinases by A. niger, currently being industrialized, has shown significant economical advantages compared to existing processes (deep tank, surface or Koji fermentation).
The object of the proposed research is to explore the most promising developments in solid state fermentation using sugar beet pulp as a substrate. The main research programme will involve microbiology, fermentation process optimization, engineering and nutritional tests. It will be conducted mainly at pilot plant scale. The anticipated outlets are in the improvement of feed composition for monogastric and polygastric animals, saccharification, protein enrichment and probiotics, and in the production of secondary metabolites such as enzymes, vitamins and amino acids.
The basic purpose of the project was to apply solid state fermentation (SSF) for the manufacture of medium or high added value products. Previous experiences have shown the feasibility of such an operation for the production of protein enriched pulp and industrial enzymes. The industrial production of pectinases by Aspergillus niger has shown significant economical advantages compared to existing processes (ie deep tank, surface or Koji fermentation). Sugar beet pulp is already an excess feedstuff in some European countries. The continuing trend towards decreased dairy production in the European Community (EC) will cause further pressure on sugar beet pulp sales into this market. A new outlet is very important for the future. The object of the research is to explore the most promising developments in SSF using sugar beet pulp as a substrate.
Analytical methods for the measurement of enzyme activity were developed and various strains of fungi were screened for the production of the desired enzymes, with mutagenesis and genetic engineering being conducted to increase productivity. Optimum fermentation conditions have also been studied for the different microorganisms.
Solid state fermentation (SSF), using sugar beet pulp as the main substrate, is being developed for the manufacture of medium of high added value products. The production of industrial enzymes to be used for animal feed improvement and other applications is under study. Analytical methods for the measure of enzyme activity were developed. Collection strains of different species of fungi were screened for the production of the desired enzymes. For the selected strains, mutagenesis was conducted to increase the productivity. At the same time, optimum fermentation conditions have been studied for the different microorganisms: compositions of the substrate; water content; temperature; and duration. After development in flask and laboratory fermenters, experiments are being conducted in 50 l pilot fermenters. Genetical engineering was used to obtain one highly productive strain of fungus.
The production of protein enriched beet pulp and probiotic enriched beet pulp is under study. For both studies, suitable strains were selected, and the nutritional quality of fermented beet pulp was evaluated. Fermentation media and fermentation conditions were developed from flask to laboratory fermenters.
Animal feed evaluation and an animal feed experiment was undertaken. In the experiment on broiler chickens positive results due to the addition of enzymes were observed.
The main advantages are the following:
high volumetric productivity due to the high concentration of substrate per unit volume of fermenter;
low capital investment;
facility of product recovery and drying;
reduction of chemical and biological oxygen demand;
possibility to recover the secondary metabolites at a high concentration;
low energy consumption.
Sugar beet pulp is an excellent substrate for SSF:
it has a satisfactory composition and is a suitable carbon source for microbial utilization;
it has a rigid and open structure allowing a specific reactor design;
pulp particles are loose and not sticky;
it has a high water holding capacity allowing solubilization of nutrients;
it is available in large quantities at low cost.
Sugar beet pulp is already an excess feedstuff in some European countries. The continuing trend for decreased dairy production in the EC will cause further pressure on sugar beet pulp sales into this market. A new outlet is very important for the future.
Ámbito científico (EuroSciVoc)
CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural. Véas: El vocabulario científico europeo..
CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural. Véas: El vocabulario científico europeo..
- ciencias naturales ciencias biológicas microbiología micología
- ciencias agrícolas agricultura, silvicultura y pesca agricultura cultivos industriales forraje
- ciencias naturales ciencias biológicas bioquímica biomoléculas proteínas enzima
- ingeniería y tecnología biotecnología industrial tecnología de bioprocesos fermentación
- ciencias agrícolas ciencias animales y lácteas mascotas ganadería piensos para animales
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Programa(s)
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Programas de financiación plurianuales que definen las prioridades de la UE en materia de investigación e innovación.
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Las convocatorias de propuestas se dividen en temas. Un tema define una materia o área específica para la que los solicitantes pueden presentar propuestas. La descripción de un tema comprende su alcance específico y la repercusión prevista del proyecto financiado.
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Procedimiento para invitar a los solicitantes a presentar propuestas de proyectos con el objetivo de obtener financiación de la UE.
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Régimen de financiación (o «Tipo de acción») dentro de un programa con características comunes. Especifica: el alcance de lo que se financia; el porcentaje de reembolso; los criterios específicos de evaluación para optar a la financiación; y el uso de formas simplificadas de costes como los importes a tanto alzado.
Coordinador
75008 Paris
Francia
Los costes totales en que ha incurrido esta organización para participar en el proyecto, incluidos los costes directos e indirectos. Este importe es un subconjunto del presupuesto total del proyecto.