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APPLICATIONS OF BIOTECHNOLOGY FOR REDUCED INPUTS IN DISEASE PROTECTION AND NITROGEN FERTILIZATION OF RAPESEED CULTIVATION

Objective

The present state of biotechnological knowledge in rapeseed is ready for important applications such as lowering the inputs in rapeseed productions.

The Concerted Action is proposed to strengthen ongoing molecular and genetic research work to further increase the ecological compatibility and to reduce the production costs of rapeseed by increasing the nitrogen uptake and nitrogen use efficiency as well as by improving genetic mechanisms of disease resistance.

For Phoma lingam, the most severe fungal rapeseed disease, different molecular approaches to tag and to isolate the plant resistance genes have been started in all three countries of the present applicants. Within the Concerted Action the different approaches will be compared, information exchanged and further experimental work will be coordinated.

Nitrogen uptake and nitrogen use efficiency being another critical point in ecological rapeseed production is the second target of mutual interest, that is very complex and known for long. Strategies to tackle this problem are just now being started. Within the proposed Concerted Action the activities of the participating research groups will be organised. Work will be directed to new informations about metabolic control points of N-metabolism in rapeseed by performing transformation experiments with genes of critical enzymes in the nitrogen pathway.

The large efforts made to isolate various plant genes of agricultural importance have provided many promising gene constructs which are now available for testing. However, a bottleneck for further utilisation of the gene constructs is the transformation of important breeding lines and cultivars. Considering the increasing public awareness of ecological risks, transformation methods of interest are those which allow integration of 'genes of interest' and the 'selectable marker gene' at different positions in the genome in order to facilitate the subsequent elimination of the marker in segregating offsprings. Relevant approaches are co-transformation of explants by A. rhizogenes and A. tumefaciens, and of microspores and microspore-derived embryoids by electroporation and use of the particle gun. This work is the third part of the proposed Concerted Action.

Funding Scheme

CON - Coordination of research actions

Coordinator

Georg-August-Universität Göttingen
Address
Von-siebold-straße 8
37075 Göttingen
Germany

Participants (5)

Institut National de la Recherche Agronomique (INRA)
France
Address
Route De Saint-cyr
78000 Versailles
Institut National de la Recherche Agronomique (INRA)
France
Address
Route De Saint Cyr
78026 Versailles
JOHANNES GUTENBERG UNIVERSITAET MAINZ
Germany
Address
Obere Zahlbacher Strasse 67
55131 Mainz
NOPA / NOeMI Research Unit
France
Address
Inra, Blg 440
78352 Jouy-en-josas
SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES
Sweden
Address
Box
75007 Uppsala