Development of Sensors for On-Site Monitoring of the Extent of Pollution in Water Resources

Información del proyecto

Identificador del acuerdo de subvención: AVI*930002

Proyecto cerrado

Fecha de inicio 1 Mayo 1994

Fecha de finalización 31 Diciembre 1997

Financiado con arreglo a

• Avicenne Initiative (Science and technology cooperation with the Maghreb and the countries of the Mediterranean Basin), 1991-

Coste total

Sin datos

Aportación de la UE

Sin datos

Coordinado por

University of Newcastle upon Tyne
United Kingdom

Objetivo

- To develop a system for monitoring the extent of pollution of water resources based on low-cost, highly sensitive amperometric biosensor instrumentation that utilises biological recognition components (chelators and antibodies) in intimate contact with electrode surfaces for signal transduction and pollutant determination. The pollutants which will be considered are heavy metal ions, pesticides and bacteria;
- To develop a user-friendly, compact and reliable instrument for field use.
- The major objective of identification and synthesis of compounds that displayed suitable electrochemistry to be used as specific chelators for heavy metal ions resulted in the development of electrodes for Cu, Cd, Al and Pb. These are presently being incorporated into the disposable electrodes by both screen printing and ink-jet printing techniques and are available for in-field testing.
- A second major objective has seen the development of a one-step, separation-free, electrochemical immunosensor system for the bacteria Escherichia coli c600, Salmonella typhimurium and Bacillus subtilis. These have been demonstrated and have been adapted for use in conjunction with disposable screen-printed electrodes. This approach has been published in Biosensors and Bioelectronics.
- In a third major objective, a new immunosensor system for one-step immunoassay of pesticides such as atrazine has been developed. The immunosensor employs a competitive immunoassay with glucose oxidase-pesticide conjugates and direct electron transfer to horseradish peroxidase immobilised at an electrode surface.
- Electrochemical biosensor instrumentation has been constructed and is undergoing evaluation in Malta.
- Design, synthesis and testing of metal-specific complexing agents suitable for immobilisation on printed disposable electrodes for voltammetric analysis of heavy metal pollution;
- Development of electrochemical immunosensors for measurement of pesticide and bacterial contamination. The biological recognition elements (antibodies) were to be immobilised at or near the surface of disposable electrodes and two approaches to electrochemical detection of enzyme label activity assessed and optimised. Horseradish peroxidase to be used as the enzyme label for bacterial immunosensors and glucose oxidase for pesticide immunosensors;
- Manufacture of generic disposable printed electrodes which could be employed in all aspects of the project simply by changing the surface recognition components;
- Development of low-cost instrumentation.

Ámbito científico (EuroSciVoc)

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Programa(s)

• IC-AVICENNE - Avicenne Initiative (Science and technology cooperation with the Maghreb and the countries of the Mediterranean Basin), 1991-

Tema(s)

Datos no disponibles

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

CSC - Cost-sharing contracts

Coordinador

University of Newcastle upon Tyne

• Aportación de la UE: Sin datos
• Dirección: 6 Framlington Place ; NE2 4HH Newcastle upon Tyne ; Reino Unido
• Coste total: Sin datos

Participantes (2)

TEL AVIV UNIVERSITY

Israel

• Aportación de la UE: Sin datos
• Dirección: Ramat Aviv ; 69978 RAMAT - AVIV
• Coste total: Sin datos

University of Malta

Malta

• Aportación de la UE: Sin datos
• Dirección: MSD 08 Msida
• Coste total: Sin datos