Objective
MANIPULATION OF PLANT BEHAVIOUR BY GENE TRANSFER DEPENDS UPON KNOWING HOW THE EXPRESSION OF GENES IS CONTROLLED, AS WELL AS IDENTIFYING THE ACTION OF PARTICULAR GENES.
THE FOLLOWING OBJECTIVES :
- UNDERSTANDING THE MECHANISMS OF THE CONTROL OF GENE EXPRESSION IN PLANTS;
- OBTAINING A RANGE OF CONTROL SEQUENCES THAT CAN BE USED TO DIRECT THE EXPRESSION OF CERTAIN STRUCTURAL GENES, INCLUDING THOSE REGULATING PLANT DEVELOPMENT;
ARE FUNDAMENTAL TO ALL ASPECTS OF THE GENETIC MANIPULATION OF CROP PLANTS, AND PROGRESS IN THAT ENTIRE RESEARCH AREA WILL BE LIMITED BY THE POSSIBILITIES TO HAVE THEM FULFILLED.
The mechanisms controlling gene expression in plants were investigated. Specifically, 4 important cereal seed protein genes and 3 differentially regulated genes coding for glutamine synthetase (a key enzyme in the assimilation of nitrogen by plants) from French bean were examined.
The control regions of 4 cereal seed protein genes, 3 differentially expressed glutamine synthetase genes and the T-cyt gene of A tumefaciens were characterized.
Genes related to plant growth and development were isolated from potato, barley, maize and tobacco, including genes that are activated by the plant growth regulating auxins and cytokinins (plant hormones) as well as genes that are developmentally controlled in maize and barley.
Agrobacterium Ri and Ti T-deoxyribonucleic acid (T-DNA) genes were found to give a spectrum of changes in growth and development in transgenic potato, including effects on the development of root systems. The natural Agrobacterium gene transfer system was studied in detail and genes determining host range and efficiency of transfer were characterized. Gene transfer techniques were also developed for rice.
THE WORK WILL HAVE THE FOLLOWING SPECIFIC AIMS :
- ISOLATION AND CHARACTERIZATION OF CONTROL REGIONS OF GENES (MAINLY THE LABORATORY OF HARPENDEN);
- DEVELOPMENT OF SYSTEMS FOR THE RAPID FUNCTIONAL ANALYSIS OF PUTATIVE CONTROL REGIONS (BOTH LABORATORIES);
- IDENTIFICATION AND CHARATERIZATION OF GENES INVOLVED IN PLANT GROWTH REGULATION (BOTH LABORATORIES).
UPSTREAM SEQUENCES OF IDENTIFIED GENES WILL BE ISOLATED AND ANALYZED BOTH FROM STRUCTURAL AND FUNCTIONAL POINTS OF VIEW. SYSTEMS FOR THE STUDY OF TRANSIENT EXPRESSION OF TRANSFORMED GENES IN PROTOPLASTS AND/OR CELLS WILL BE DEVELOPED.
THE EFFECT OF TI- AND RI-PLASMIDS DERIVED ONC GENES ON THE BEHAVIOUR OF TRANSFORMED ENTITIES WILL BE STUDIED, AND GENES INVOLVED IN REGULATION OF GROWTH, WHICH THE PLANT ITSELF HARBOURS, WILL BE SEARCHED AND CHARATERIZED.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
2300 RA LEIDEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.