Skip to main content
Go to the home page of the European Commission (opens in new window)
English English
CORDIS - EU research results
CORDIS
Content archived on 2024-04-15

HIGH LEVEL EXPRESSION OF FOOT-AND-MOUTH DISEASE VIRUS ANTIGENS USING A BACULOVIRUS VECTOR

Objective


Research has been undertaken to develop a system, utilising highly efficient baculovirus vectors, for making foot and mouth disease (FMD) virus procapsids as the basis of a safe and effective vaccine. Four discrete antibody binding regions on FMD virus were identified. A complementary deoxyribonucleic acid (cDNA) clone of the type C procapsid was prepared. The functions of the 3 virus protease genes were studied by constructing cassettes of serotype O or A procapsid genes with various combinations of protease genes, and expressing them in a cell free translation system or in mammalian cells. Several were also made into baculovirus vectors, including one carrying all 3 protease genes. The latter, when used to infect insect cells, yielded small amounts of FMD virus protein able to self process to procapsid subunits, some of which then assembled into procapsid like structures. Difficulties encountered in trying to increase expression were traced to the toxic L protease, a problem that could be overcome by making deletions in the L gene. A new type of baculovirus vector, identifiable by a simple colour test and able to infect insect larvae, was developed.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.

You need to log in or register to use this function

Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Data not available

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

Data not available

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

CSC - Cost-sharing contracts

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
EU contribution
No data
Address
Serrano 117
MADRID
Spain

See on map

Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data
My booklet 0 0