Objectif Research has been undertaken to develop a system, utilising highly efficient baculovirus vectors, for making foot and mouth disease (FMD) virus procapsids as the basis of a safe and effective vaccine. Four discrete antibody binding regions on FMD virus were identified. A complementary deoxyribonucleic acid (cDNA) clone of the type C procapsid was prepared. The functions of the 3 virus protease genes were studied by constructing cassettes of serotype O or A procapsid genes with various combinations of protease genes, and expressing them in a cell free translation system or in mammalian cells. Several were also made into baculovirus vectors, including one carrying all 3 protease genes. The latter, when used to infect insect cells, yielded small amounts of FMD virus protein able to self process to procapsid subunits, some of which then assembled into procapsid like structures. Difficulties encountered in trying to increase expression were traced to the toxic L protease, a problem that could be overcome by making deletions in the L gene. A new type of baculovirus vector, identifiable by a simple colour test and able to infect insect larvae, was developed. Champ scientifique ingénierie et technologieingénierie des materiauxcouleurssciences naturellessciences biologiquesmicrobiologievirologiesciences naturellessciences biologiquesgénétiqueADNsciences médicales et de la santémédecine fondamentalepharmacologie et pharmacieproduit pharmaceutiquevaccinssciences naturellessciences biologiqueszoologieentomologie Programme(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Adresse Serrano 117 Madrid Espagne Voir sur la carte Contribution de l’UE Aucune donnée
