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Content archived on 2024-04-15

MUTAGENIC EFFECTS OF IONIZING RADIATION IN BACTERIA AND MAMMALIAN CELLS

Objective

EVALUATION OF GENETIC RISKS FROM IONIZING RADIATION. DETERMINATION OF CELLULAR RADIOSENSITIVITY AND RELATION TO REPAIR.
The molecular specificity of radiation induced genetic alterations in Escherichia coli has been studied and an assessment made of the risk for humans.

The objectives were focused on: the genetic characterisation of the mechanism of SOS mutagenesis using different mutants of E. coli; the relationship between DNA replication fidelity, mismatch repair and the presence of lesions in the DNA; the replication and mutagenesis of damaged DNA using in vitro damaged heteroduplex DNA: the study of the conversion of premutagenic lesions into mutations, in specific sequences of DNA; several factors govern the conversion of premutagenic lesions into mutations including reactivity of DNA bases, sequence context and the local configuration of the DNA Helix; the identification of E. coli proteins involved in mutagenesis and able to recognize specific local configuration.
MOLECULAR SPECIFICITY OF RADIATION-INDUCED GENETIC ALTERATIONS IN E COLI: RISK ASSESSMENT FOR HUMANS.

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Coordinator

UNIVERSITE LIBRE DE BRUXELLES
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AV. F.D. ROOSEVELT,50
1050 BRUSSELS
Belgium

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