Objective
The object of this research was to refine further the techniques of human sperm chromosome analysis, based on the in vitro fertilisation of zona free hamster eggs, by the use of electropermeabilisation for the induction of a functional acrosome reaction in the spermatozoa and the use of a totally defined medium for gamete culture. This adapted technique was applied to an investigation of the effect of radiation on the induction of cytogenetic aberration.
RESEARCH INTENDS TO STUDY DIRECTLY THE EFFECTS OF RADIATION ON THE INDUCTION OF CHROMOSOME ABNORMALITIES IN HUMAN SPERM TAKING INTO ACCOUNT PARAMETERS SUCH AS AGE, DISEASE, ENVIRONMENTAL AND PHYSIOLOGICAL CONDITIONS.
EXPECTED BENEFITS
EVALUATION OF GENETIC RISKS FROM IONIZING RADIATION. DETERMINATION OF GENETIC DAMAGE IN MAN ON THE BASIS OF CHROMOSOME ABERRATIONS.
Fields of science
Topic(s)
Data not availableCall for proposal
Data not availableFunding Scheme
CSC - Cost-sharing contractsCoordinator
Ireland