Skip to main content

INDUCTION OF MYELOID LEUKAEMIA IN MICE BY IRRADIATION WITH FISSION NEUTRONS AS A FUNCTION OF DOSE RATE

Objective



IN 1982 MOLE AND DAVIDS HAVE REPORTED ON THE INDUCTION OF MYELOID LEUKAEMIA IN MICE BY IRRADIATION WITH FISSION NEUTRONS IN THE LOW FLUX REACTOR (LFR) AT PETTEN. ACUTE MYELOID LEUKAEMIA WAS INDUCED IN MALE CBA/H MICE BY EACH OF SEVEN DOSES IN THE 0,002-2 GY RANGE. A GOOD QUANTITATIVE FIT TO THE DATA WAS PROVIDED BY POSTULATING THAT CELLULAR TRANSFORMATION (INDUCTION) OF MYELOID LEUKAEMIA IS LINEAR WITH DOSE AND THAT THE FREQUENCY OF OVERT LEUKAEMIA IS LESS THAN THE FREQUENCY OF INDUCTION BECAUSE SURVIVAL OF TRANSFORMED CELLS IS DOSE DEPENDENT. THE ACUTE MYELOID LEUKAEMIA WAS DIAGNOSED BY STRINGENT CRITERIA.
A SECOND EXPERIMENT WAS PLANNED IN ORDER TO STUDY THE EFFECT OF NEUTRON DOSE-RATE ON THE INDUCTION OF ACUTE MYELOID LEUKAEMIA. A FIXED FISSION NEUTRON DOSE OF 0,4 GY WAS GIVEN TO THREE GROUPS, EACH OF ABOUT 90 CBA/H MICE AT THREE DIFFERENT DOSE RATES EQUAL TO 0,1 GY, 0,01 GY AND 0,02 GY PER MINUTE RESPECTIVELY. THESE THREE GROUPS TOGETHER WITH AN UNIRRADIATED CONTROL GROUP OF EQUAL NUMBER HAVE BEEN KEPT DURING THEIR LIFE SPAN IN THE PETTEN LABORATORY. CAGES WERE EXAMINED DAILY FOR DEAD AND AFFECTED ANIMALS. THE LATTER WERE DELIBERATELY KILLED. AT THE STANDARD POST-MORTEM EXAMINATION AND EMBEDDED IN PARAFFIN BLOCKS. THERE WERE NO OUTBREAKS OF EPIDEMIC DISEASE OR OTHER UNTOWARD PHENOMENA AFFECTING ANIMAL SURVIVAL.
THE RESEARCH PROPOSAL COMPRISES ONE PROJECT WHICH INCLUDES:
1. THE PREPARATION OF THE HISTOLOGICAL MATERIAL;
2. THE EXAMINATION OF THE HISTOLOGICAL SPECIMENS IN ORDER TO DIAGNOSE MYELOID LEUKAEMIA;
3. THE CONSTRUCTION OF CUMMULATIVE SURVIVAL CURVES FOR ALL GROUPS;
4. ANALYSIS OF THE DATA;
5. PUBLICATION OF THE RESULTS.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

STICHTING ENERGIE ONDERZOEK CENTRUM NEDERLAND
Address
Westerduinweg 3
2584 AE Den Haag
Netherlands