Objective
The medium-term objective is to determine, before the end of 1997, the entire genome sequence of Bacillus subtilis, which is both of industrial importance and a model organism for Gram-positive bacteria. An international agreement has been reached allowing the allocation of regions of the chromosome among the groups of the European and Japanese Bacillus subtilis genome sequencing networks. This international project currently includes 18 European and 7 Japanese laboratories, together with 2 biotechnology companies (Genencor International and Novo Nordisk) and 1 Korean laboratory.
The European sequencing groups have obtained support from the European Commission (this contract and contract BIO2 CT-942011). The initial objective of contract BIO2 CT-930272 was to determine the DNA sequence of about 23% (0.97 Mbp) of the Bacillus subtilis genome.
A cloning strategy for the construction of gene banks representative of the entire Bacillus subtilis chromosome is not available. This is, at least in part, due to the generally high level of expression of Bacillus subtilis genes observed in Escherichia coli, leading to toxic effects in this commonly used cloning host. However, participating groups have employed a variety of strategies to overcome this limitation. They include: (1) cloning into a range of plasmid, lambda and YAC vectors; (2) the use of an Escherichia coli strain that lowers the copy number of ColE1-based plasmids and which correspondingly reduces the potential toxic effects of cloned genes; (3) the use of integrative plasmids and marker rescue in genome walking experiments; (4) in vitro amplification using standard, long-range (LR PCR) and inverse PCR techniques.
MAJOR SCIENTIFIC BREAKTHROUGHS AND INDUSTRIAL APPLICATIONS:
During this contract 1.16 Mbp of Bacillus subtilis DNA has been sequenced. The European sequencing network has presently deposited a total of 1.9 Mbp of new Bacillus subtilis DNA sequence at the database maintained by the Institut Pasteur. In the current EU framework, a relational database containing 3.6 Mbp of non-redundant Bacillus subtilis DNA sequences, representing 86% of the total genome, has been constructed at the Institut Pasteur. The data are available at a new World Wide Web site: http://www.pasteur.fr/Bio/SubtiList.html(opens in new window).
An industrial platform has been set up to facilitate contacts between project participants and European biotechnology companies. The following companies have agreed to this mode of cooperation: DuPont de Nemours (France, USA), Genencor (Finland, USA), F. Hoffmann-La Roche AG (Switzerland), Novo Nordisk (Denmark), Frimond (Belgium), Roussel Uclaf (France), SmithKline Beecham (United Kingdom). Moreover, a confidential document containing a list of similarities of putatively identified B. subtilis proteins with known gene products is forwarded by the coordinator, at regular intervals, to the companies mentioned above.
Fields of science (EuroSciVoc)
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
- natural sciences computer and information sciences internet world wide web
- natural sciences computer and information sciences databases relational databases
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75724 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.