CORDIS provides links to public deliverables and publications of HORIZON projects.
Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .
Deliverables
Summary:
A bovine large-insert DNA library was constructed in a Bacterial Artificial Chromosome (BAC) vector. A total of 157,240 BACs have been picked into 384-well plates. Approximately 190 randomly selected clones were sized by Pulsed Field Gel electrophoresis, and have an average insert size of 105 kilobases. The library thus represents 5-6 genome equivalents and will give greater than 99% chance of recovering any given single-copy sequence. The frequency of clones without inserts is 4%.Clones from 360 x 384-well plates were gridded onto high-density nylon membranes. Superpools for PCR-screening were produced from the same set of clones. Both membranes and superpools are available from Laboratory 4 (MPIMG-Berlin)
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