Development and application of fine-mapping methods for the study of ETL in cattle

Informazioni relative al progetto

BOREALIS

ID dell’accordo di sovvenzione: BIO4950073

Progetto chiuso

Data di avvio 1 Gennaio 1996

Data di completamento 31 Dicembre 1998

Finanziato da

• Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998

Costo totale

Nessun dato

Contributo UE

Nessun dato

Coordinato da

BBSRC Babraham Institute
United Kingdom

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Risultati finali

Construction of a bovine BAC library

Summary: A bovine large-insert DNA library was constructed in a Bacterial Artificial Chromosome (BAC) vector. A total of 157,240 BACs have been picked into 384-well plates. Approximately 190 randomly selected clones were sized by Pulsed Field Gel electrophoresis, and have an average insert size of 105 kilobases. The library thus represents 5-6 genome equivalents and will give greater than 99% chance of recovering any given single-copy sequence. The frequency of clones without inserts is 4%.Clones from 360 x 384-well plates were gridded onto high-density nylon membranes. Superpools for PCR-screening were produced from the same set of clones. Both membranes and superpools are available from Laboratory 4 (MPIMG-Berlin)