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Filamentous fungi as cell factory for the production of industrial importation products

Objectif



Filamentous fungi have been successfully exploited for many years by the European fermentation industries for the production of both low molecular weight products such as organic acids, antibiotics, carotenoids and high molecular weight products including a broad spectrum of fungal enzymes. To improve the efficiency of production and quality of these compounds, and to produce new industrially important products, it is necessary to understand the biological details of why fungal cells are productive and to design efficient and reliable industrial processes based on the concept of utilising FILAMENTOUS FUNGI AS CELL FACTORIES.
This research proposal describes a highly coordinated and inter- dependent research programme with the general objective of investigating the biological and biochemical engineering components of the Filamentous-Fungal Cell Factory. Research effort is focused on the following main objectives: 1) to extend our knowledge of the genetics and cell biology of fungi to facilitate the design of fungal cells able to carry out industrially important processes; 2) to characterize metabolic pathways and fluxes, to model metabolism, identify bottlenecks and subsequently redesign and engineer metabolism; 3) to improve the efficiency of protein production and the quality of the product; 5) to characterize and subsequently manipulate secondary metabolism and to establish its interaction with primary metabolism; and 5) to develop continuous flow and fed-batch culture fermentation protocols that optimize the production of proteins. During the programme the following key issues will be studied and improved: i) nutrient uptake and signal transduction; ii) control of gene- expression; iii) primary metabolism and metabolic flux engineering; iv) secondary metabolism, metabolite synthesis and excretion; v) morphology; vi) protein secretion; vii) fermenter technology, continuous flow and fedbatch fermentation.
The programme will be carried out by thirty eight key laboratories both academic (28) and industrial (10) from ten european countries with complementary expertise in cell and molecular biology, genetics, fungal physiology, biochemistry, biophysics, biomathematics and biochemical engineering. It is expected that the combined research efforts of these laboratories will provide new and improved strategies facilitating the development and/or improvement of bioprocesses to produce various kinds of low and high molecular-weight compounds at an industrial scale. This will be beneficial for the competitiveness of the European Biotechnology Companies, which are practically all represented in this programme either as participant or as a member of the Filamentous-Fungal Industrial Platform. This platform, of which seventeen European Industries are a member, will support and guide the programme and will furthermore be involved in the exploitation of the results to be achieved.

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Coordinateur

Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek
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Adresse
48,Utrechtseweg
3700 AJ Zeist
Pays-Bas

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