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Extremophiles as cell factories

Objective



Summary Extremophilic archaea and bacteria have tremendous potential in biotechnology because of their ability to grow under extreme conditions and to produce unique stable products with high technological value. A number of enzymes from hyperthermophiles like Pyrococcus and Thermococcus, that are involved in DNA processing, are already on the market. The objective of this integrated project is aiming at the production of industrial enzymes (e.g. hydrolases and DNA polymerases) and other products (organic solutes and lipids) from selected extremophiles. Research work will be concentrating on few model organisms including hyperthermophiles (Pyrococcus, Thermococcus, Pyrodictium), extreme thermophiles (Thermotoga, Rhodothermus, Sulfolobus), psychrophiles ( Vibrio) and halophiles (Marinococcus). In order to overcome the limited production of enzymes and other organic compounds and in order to tailor these for specific applications the proposed project will be performed in an interdisciplinary manner involving (a) genetic engineering, (b) fermentation technology and (c) product engineering.
Various strategies will be used with the aim of optimization of growth of selected extremophiles. A special pilot plant bioreactor will be constructed which will allow a high mass transfer capacity between liquid and gaseous phase. Specific attention will be given to the development of expression systems for hyperthermophiles, thermoacidophiles and halophiles. The overexpression of novel products in extreme thermophiles and mesophiles will allow for further exploitation of the potential of these microbes. The analysis of structure-function relationships, the clarification of stability and specificity and catalysis mechanism will be needed to use enzymes from extremophiles in diagnostics, molecular biology and other industrial processes.

Coordinator

Technische Universitat Hamburg-Harburg
Address
Denickestraße 15
21071 Hamburg
Germany

Participants (39)

Bioengineering AG
Switzerland
Address
7,Sagenrainstraße
8636 Wald
Bohringer Mannheim GmbH
Germany
Address
Nonnenwald 2
82377 Penzberg
CHRISTIAN-ALBRECHTS UNIVERSITAET ZU KIEL
Germany
Address
1-9,Am Botanischen Garten 1-9
24118 Kiel
Centre National de la Recherche Scientifique
France
Address

29682 Roscoff
Centro de Neurosciencias de Coimbra
Portugal
Address
Largo De Marques De Pombal
3000 Coimbra
GENENCOR INTERNATIONAL BV
Netherlands
Address
139,Lange Kleiweg 139
2288 GJ Rijswijk Zh
Genis Hf
Iceland
Address
Keldnaholt
112 Reykjavik
IFREMER - INSTITUT FRANÇAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER
France
Address
Technopôle De Brest - Iroise
29280 Plouzane
KAROLINSKA INSTITUTE
Sweden
Address
Haelsvaegen 7
141 57 Huddinge
Kobenhavns Universitet
Denmark
Address
83 H,soelvgade
1307 København K
LUND UNIVERSITY
Sweden
Address
60,Chemical Center
221 00 Lund
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
Am Klopferspitz 18 A
82152 Martinsried
MERLIN Gesellschaft fur Mikrobiologische Diagnostika mbH
Germany
Address
14,Kleinstraße
53332 Bornheim-hersel
MICROBIOLOGICAL RESEARCH AUTHORITY
United Kingdom
Address
Porton Down
SP4 0JG Salisbury
National Technical University of Athens
Greece
Address

15700 Athens
National and Kapodestrian University of Athens
Greece
Address
Panepistimiopolis - Kouponia
15701 Athens
Novo-Nordisk A/S
Denmark
Address
1Bs,novo Alle
2880 Bagsværd
Osterreichische Gesellschaft fur Holzforschung
Austria
Address
7,Franz-grill-straße
1031 Wien
Primm Srl
Italy
Address
Via Olgettina 58
20132 Milano
TECHNOLOGICAL INSTITUTE OF ICELAND
Iceland
Address
Keldnaholt
112 Reykjavik
Technische Universitat Graz
Austria
Address
12,Petersgasse
8010 Graz
UNIVERSIDAD DE SEVILLA
Spain
Address
S/n,profesor Garcia Gonzalez S/n
41012 Sevilla
UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II
Italy
Address
Via Mezzocannone 16
80134 Napoli
UNIVERSITE DE PARIS-SUD XI
France
Address
Rue Georges Clemenceau 15, Bftiment 400
91405 Gometz La Ville
UNIVERSITY OF GRONINGEN
Netherlands
Address
30,Kerklaan 30
9751 NN Haren Gn
UNIVERSITY OF LEICESTER
United Kingdom
Address
Maurice Shock Building
LE1 9HN Leicester
UNIVERSITY OF LUEBECK
Germany
Address
Ratzeburger Allee 160
Luebeck
Universidade Nova de Lisboa
Portugal
Address
6,Quinta Do Marqués
2780 Oeiras
Universitat Gesamthochschule Essen
Germany
Address
5,Universitätstraße
45117 Essen
Universitat Regensburg
Germany
Address
31,Universitätstraße
93053 Regensburg
University College Dublin
Ireland
Address
Belfield
4 Dublin
University College London
United Kingdom
Address
Gower Street
WC1E 6BT London
University of Iceland
Iceland
Address
1A,armuli
0108 Reykjavik
University of Ioannina
Greece
Address
Panepistemiopolis, Dourouti
45110 Ioannina
University of Sheffield
United Kingdom
Address
Western Bank
S10 2UH Sheffield
Universitá degli Studi di Napoli Federico II
Italy
Address
Via Costantinopoli
80138 Napoli
Universitá degli Studi di Roma 'La Sapienza'
Italy
Address
Piazzale Aldo Moro
00185 Roma
WAGENINGEN UNIVERSITY
Netherlands
Address
4,Hesselink Van Suchtelenweg 4
6703 CT Wageningen
Westfälische Wilhelms-Universität Münster
Germany
Address
Wilhelm-klemm-straße 2
48149 Münster