Objective
The objective of this proposal is to sequence about 4Mb of DNA, amounting to between 3-4 per cent of the Drosophila genome.
This is to be seen as part of a larger strategy for the sequencing of the entire genome in a manner agreed between Drosophila laboratories in North America and Europe. Drosophila is a model multicellular eukaryote through which many conserved biological processes have been identified, particularly signal transduction pathways and developmental controls which are known to be operative in man. Thus a knowledge of the genetic makeup of this organism will be invaluable as a model system in which to elucidate the function and regulation of genes newly discovered in genome projects, including the human genome project. Furthermore, it will greatly enhance the utility of Drosophila as a model system in the direct development of control strategies aimed at a variety of agricultural pests and vectors of disease.
The proposed work is based on a physical map of the Drosophila genome in the form of an ordered array of overlapping recombinant cosmids, constructed by an ongoing project initiated in 1988 with continued support from the European Community.
Several hundred cosmids have been identified that correspond to the first three cytogenetic divisions of the X-chromosome. These fall into a set of 37 `contigs' that will be the basis for this sequencing project. The manner in which these cosmids were selected identified these contigs as sets of related overlapping clones, which will have to be converted into a linear physical array, before sequencing may be initiated. Gaps between cosmid contigs will be linked using other cosmid and P1 clones in a PCRbased strategy by the co-ordinating laboratory in Dundee.
Cosmids will be distributed to five sequencing laboratories, whose work will be monitored and sub-coordinated by the group at the Sanger Centre. Assembled sequence will be sent to the Cambridge laboratory for analysis using gene prediction programmes; for correlation with the extensive knowledge of the genetics and chromsosomal structure for this region; and for incorporation into the extensive Drosophila genetic database that is being developed.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
- natural sciencescomputer and information sciencesdatabases
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesgeneticsgenomes
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Topic(s)
Call for proposal
Data not availableFunding Scheme
CSC - Cost-sharing contractsCoordinator
DD1 4HN Dundee
United Kingdom