This project brings together the most active groups working on bacterial pathogenesis within the EU. The common interest of the partners in this project is to examine the delivery of elicitors and pathogenicity factors and their interaction with plant cells. The bacterial pathogens to be studied, Xanthomonas campestris pv. vesicatoria (Xcv), Pseudomonas syringae pv. phaseolicola (Psph) and Ralstonia (pseudomonas) solanacearum (Rsol), cause economically important diseases. Rsol is a vascular pathogen with a remarkably wide host range and provides an important comparison with Xcv and Psph which primarily invade the intercellular spaces in leaf parenchyma. The proposal is based on seven interactive work packages on the following topics:
1. Molecular dissection of hrp gene structure and function.
2. Determination of the function of avirulence (avr) genes from Xcv and Psph. 3. Analysis of the bacterial surface structures involved in signal delivery to plant cells.
4. Identification of proteins (pathogenicity factors)secreted by the hrp dependent type III pathway.
5. Characterization of plant receptors for bacterial pathogenicity factors and elicitors of the hypersensitive reaction.
Industrial partners will integrate with the basic studies to examine:
6. The effect of hrp genes on xanthan production.
7. Rational selection of chemicals for crop protection and antimicrobial activity.
The objectives implicit in these work packages exactly match the research tasks specified in the work programme for Plant Molecular and Cellular Biology 3.1.3. Resistance to stress and pathogens namely:
- Isolation of bacterial genes coding for pathogenesis factors and elicitor synthesizing enzymes.
- Studies to reveal the nature of transduction pathways leading to disease resistance.
Funding SchemeCSC - Cost-sharing contracts
91190 Gif Sur Yvette
06120 Halle (Saale)
TN25 5AH Wye