Objective
A multitude of techniques has become available in recent times to isolate differentially expressed genes. Among these, random primed RNA fingerprinting is a powerful tool for the isolation of coding regions of transcriptionally regulated genes. The main objective of the proposed work programme is to evaluate the efficiency and the feasibility of a computer-assisted approach for searching a new set of RNA fingerprinting 12-mers (DNA primers) to develop a new protocol of cDNA fingerprinting for the differential screening of gene expression. The collection of reagents generated through this approach will allow us to propose a simple, exhaustive and systematic tool for the analysis of differential gene expression as a worktable, advantageous alternative to differential display techniques. The aim is to develop a marketable product for the differential screening of gene expression adapted to both manual operations and automatic workstations. The activity will concern theoretical PCR simulations performed on selected non-redundant databases to isolate a set of 12-mer oligonucleotides with particular thermodynamic parameters, the evaluation of the efficiency and specificity of the chosen primers by PCR amplification experiments on RNA extracted from tissues and the sequence analysis of cloned products. Preliminary studies, performed on a partial cDNA library, makes feasible the construction of different panels of 96 primers displaying marked affinity for coding portions of known genes and, presumably, of novel one, and useful for the development of micro-titer-plate commercial kits.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
MILANO
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.