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Content archived on 2024-05-14

Cultivation of epi- and endothelial cells on biomimetic polymer surface deriving from molecular imprint of flat fibrillar collagen network

Objective



Epithelia and endothelia are specialized cellular linings along animal tissue interfaces, performing tasks like protection, permeability, secretion, and resorption. Reliable in-vitro models are yet out of reach, because this high cell diferentiation can not be fully reestablished under standard culture conditions. Adequate substrate anchorage is essential in this. In vivo, re-anchorage physiologically occurs during tissue development and repair after a phase of cell proliferation and migration is accomplished, whereby contact with underlying extracellular matrix, in particular fibrillar collagen network, is required. With regard to the need of reliability in industrial applications, an innovative approach for the generation of artificial culture substrate, the surface of which could mimic such cell environmental aspects shall be explored. Fibrillar collagen associated properties which are involved in cell adhesion and morphogensis shall be examined. They may be topological, physicochemical, and biotechemical in nature and may have various size scales. Such structural motifs are to be transferred from in-vitro formed flat fibrallar collagen networks to polymer surfaces by molecular imprinting. This method involved contact polymerization on a biological template to form a negative relief which, in turn is imprinted by another polymer, generating multiple positive molds which supposedly have a biomimetic surface. The ultrastructure of biological and artificial templates as well as transfer characteristics are to be analysed by means of atomic force and scanning electron microscopy. Biological and artificial substrat activity will be assayed in cultures of vascular endothelial and corneal epithelial cells, respectively. The biomimetic substrate shall be optimized and applied to prototype culture models, if necessary involving further surface modification. Progress is expected for basic cultivation-related animal cell biology as well as pharmaco-toxicological invitor testing.

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Coordinator

Interactiva Biotechnologie G.m.b.H.
EU contribution
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Address
10,Sedanstrasse
89077 Ulm
Germany

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Participants (2)

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